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Blood, 1 March 2006, Vol. 107, No. 5, pp. 1933-1942. Prepublished online as a Blood First Edition Paper on November 17, 2005; DOI 10.1182/blood-2005-08-3137. This Article Full Text Full Text (PDF) Supplemental Tables, Figures, and Videos All Versions of this Article: 2005-08-3137v1 107/5/1933    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, C.-N. Articles by Chiu, J.-J. Search for Related Content PubMed PubMed Citation Articles by Chen, C.-N. Articles by Chiu, J.-J. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Neutrophils, lymphocytes, and monocytes exhibit diverse behaviors in transendothelial and subendothelial migrations under coculture with smooth muscle cells in disturbed flow Cheng-Nan Chen, Shun-Fu Chang, Pei-Ling Lee, Kyle Chang, Li-Jing Chen, Shunichi Usami, Shu Chien, and Jeng-Jiann Chiu From the Institute of Life Sciences, National Defense Medical Center, National Health Research Institutes, Taiwan, Republic of China, the Division of Medical Engineering Research, National Health Research Institutes, Taiwan, Republic of China; the Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA; and the Institute of Biomedical Engineering, National Yang-Ming University, Taiwan, Republic of China. Atherosclerosis develops at regions of the arterial tree exposed to disturbed flow. The early stage of atherogenesis involves the adhesion of leukocytes (white blood cells [WBCs]) to and their transmigration across endothelial cells (ECs), which are located in close proximity to smooth muscle cells (SMCs). We investigated the effects of EC/SMC coculture and disturbed flow on the adhesion and transmigration of 3 types of WBCs (neutrophils, peripheral blood lymphocytes [PBLs], and monocytes) using our vertical-step flow (VSF) chamber, in which ECs were cocultured with SMCs in collagen gels. Such coculture significantly increased the adhesion and transmigration of neutrophils, PBLs, and monocytes under VSF, particularly in the reattachment area, where the rolling velocity of WBCs and their transmigration time were decreased, as compared with the other areas. Neutrophils, PBLs, and monocytes showed different subendothelial migration patterns under VSF. Their movements were more random and shorter in distance in the reattachment area. Coculture of ECs and SMCs induced their expressions of adhesion molecules and chemokines, which contributed to the increased WBC adhesion and transmigration. Our findings provide insights into the mechanisms of WBC interaction with the vessel wall (composed of ECs and SMCs) under the complex flow environments found in regions of prevalence for atherogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M.-L. Sung, C.-C. Wu, H.-I Chang, C.-K. Yen, H. J. Chen, J.-C. Cheng, S. Chien, and C.-N. Chen Shear Stress Inhibits Homocysteine-Induced Stromal Cell-Derived Factor-1 Expression in Endothelial Cells Circ. Res., October 9, 2009; 105(8): 755 - 763. [Abstract] [Full Text] [PDF] H. Loppnow, K. Werdan, and M. Buerke Invited review: Vascular cells contribute to atherosclerosis by cytokine- and innate-immunity-related inflammatory mechanisms Innate Immunity, April 1, 2008; 14(2): 63 - 87. [Abstract] [PDF] M. van Leeuwen, M. J.J. Gijbels, A. Duijvestijn, M. Smook, M. J. van de Gaar, P. Heeringa, M. P.J. de Winther, and J. W. C. Tervaert Accumulation of Myeloperoxidase-Positive Neutrophils in Atherosclerotic Lesions in LDLR / Mice Arterioscler Thromb Vasc Biol, January 1, 2008; 28(1): 84 - 89. [Abstract] [Full Text] [PDF] J.-J. Chiu, L.-J. Chen, C.-I Lee, P.-L. Lee, D.-Y. Lee, M.-C. Tsai, C.-W. Lin, S. Usami, and S. Chien Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stress Blood, July 15, 2007; 110(2): 519 - 528. [Abstract] [Full Text] [PDF]

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