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Blood, 1 March 2006, Vol. 107, No. 5, pp. 2153-2161. Prepublished online as a Blood First Edition Paper on November 8, 2005; DOI 10.1182/blood-2005-07-2701. This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: 2005-07-2701v1 107/5/2153    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lee, R. H. Articles by Prockop, D. J. Search for Related Content PubMed PubMed Citation Articles by Lee, R. H. Articles by Prockop, D. J. Related Collections Hematopoiesis and Stem Cells Cell Adhesion and Motility Stem Cells in Hematology Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice Ryang Hwa Lee, Shu Ching Hsu, James Munoz, Jin Sup Jung, Na Rea Lee, Radhika Pochampally, and Darwin J. Prockop From the Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA; and the Department of Physiology, College of Medicine, Pusan National University, Pusan, Republic of Korea. Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a "vascular niche." Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. H. Lee, M. J. Seo, A. A. Pulin, C. A. Gregory, J. Ylostalo, and D. J. Prockop The CD34-like protein PODXL and {alpha}6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice Blood, January 22, 2009; 113(4): 816 - 826. [Abstract] [Full Text] [PDF] T. Morito, T. Muneta, K. Hara, Y.-J. Ju, T. Mochizuki, H. Makino, A. Umezawa, and I. Sekiya Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans Rheumatology, August 1, 2008; 47(8): 1137 - 1143. [Abstract] [Full Text] [PDF] D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop Stem Cells and Cell Therapies in Lung Biology and Lung Diseases Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667. [Full Text] [PDF] N. Sessarego, A. Parodi, M. Podesta, F. Benvenuto, M. Mogni, V. Raviolo, M. Lituania, A. Kunkl, G. Ferlazzo, F. D. Bricarelli, et al. Multipotent mesenchymal stromal cells from amniotic fluid: solid perspectives for clinical application Haematologica, March 1, 2008; 93(3): 339 - 346. [Abstract] [Full Text] [PDF] R. Romieu-Mourez, M. Francois, M.-N. Boivin, J. Stagg, and J. Galipeau Regulation of MHC Class II Expression and Antigen Processing in Murine and Human Mesenchymal Stromal Cells by IFN-{gamma}, TGF-beta, and Cell Density J. Immunol., August 1, 2007; 179(3): 1549 - 1558. [Abstract] [Full Text] [PDF] J. E. Ip, Y. Wu, J. Huang, L. Zhang, R. E. Pratt, and V. J. Dzau Mesenchymal Stem Cells Use Integrin beta1 Not CXC Chemokine Receptor 4 for Myocardial Migration and Engraftment Mol. Biol. Cell, August 1, 2007; 18(8): 2873 - 2882. [Abstract] [Full Text] [PDF] M. Shi, J. Li, L. Liao, B. Chen, B. Li, L. Chen, H. Jia, and R. C. Zhao Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice Haematologica, July 1, 2007; 92(7): 897 - 904. [Abstract] [Full Text] [PDF] G. C. Schatteman, M. Dunnwald, and C. Jiao Biology of bone marrow-derived endothelial cell precursors Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18. [Abstract] [Full Text] [PDF] D. J. Prockop MSCs roll into the mainstream Blood, December 1, 2006; 108(12): 3626 - 3627. [Full Text] [PDF] R. H. Lee, M. J. Seo, R. L. Reger, J. L. Spees, A. A. Pulin, S. D. Olson, and D. J. Prockop Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD/scid mice PNAS, November 14, 2006; 103(46): 17438 - 17443. [Abstract] [Full Text] [PDF] D. J. Weiss, M. A. Berberich, Z. Borok, D. B. Gail, J. K. Kolls, C. Penland, and D. J. 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