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Blood, 15 March 2006, Vol. 107, No. 6, pp. 2262-2270. Prepublished online as a Blood First Edition Paper on November 22, 2005; DOI 10.1182/blood-2005-08-3365. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-08-3365v1 107/6/2262    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yang, M. Articles by Odgren, P. R. Search for Related Content PubMed PubMed Citation Articles by Yang, M. Articles by Odgren, P. R. Related Collections Hematopoiesis and Stem Cells Gene Expression Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Chemokine and chemokine receptor expression during colony stimulating factor-1–induced osteoclast differentiation in the toothless osteopetrotic rat: a key role for CCL9 (MIP-1) in osteoclastogenesis in vivo and in vitro Meiheng Yang, Geneviève Mailhot, Carole A. MacKay, April Mason-Savas, Justin Aubin, and Paul R. Odgren From the Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA. Osteoclasts differentiate from hematopoietic precursors under systemic and local controls. Chemokines and receptors direct leukocyte traffic throughout the body and may help regulate site-specific bone resorption. We investigated bone gene expression in vivo during rapid osteoclast differentiation induced by colony-stimulating factor 1 (CSF-1) in Csf1-null toothless (tl/tl) rats. Long-bone RNA from CSF-1–treated tl/tl rats was analyzed by high-density microarray over a time course. TRAP (tartrate-resistant acid phosphatase)–positive osteoclasts appeared on day 2, peaked on day 4, and decreased slightly on day 6, as marrow space was expanding. TRAP and cathepsin K mRNA paralleled the cell counts. We examined all chemokine and receptor mRNAs on the arrays. CCL9 was strongly induced and peaked on day 2, as did its receptor, CCR1, and regulatory receptors c-Fms (CSF-1 receptor) and RANK (receptor activator of nuclear factor B). Other chemokines and receptors showed little or no significant changes. In situ hybridization and immunohistochemistry revealed CCL9 in small, immature osteoclasts on day 2 and in mature cells at later times. Anti-CCL9 antibody inhibited osteoclast differentiation in culture and significantly suppressed the osteoclast response in CSF-1–treated tl/tl rats. While various chemokines have been implicated in osteoclastogenesis in vitro, this first systematic analysis of chemokines and receptors during osteoclast differentiation in vivo highlights the key role of CCL9 in this process. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Onan, E. H. Allan, J. M. W. Quinn, J. H. Gooi, S. Pompolo, N. A. Sims, M. T. Gillespie, and T. J. Martin The Chemokine Cxcl1 Is a Novel Target Gene of Parathyroid Hormone (PTH)/PTH-Related Protein in Committed Osteoblasts Endocrinology, May 1, 2009; 150(5): 2244 - 2253. [Abstract] [Full Text] [PDF] A. Yano, S. Tsutsumi, S. Soga, M.-J. Lee, J. Trepel, H. Osada, and L. Neckers Inhibition of Hsp90 activates osteoclast c-Src signaling and promotes growth of prostate carcinoma cells in bone PNAS, October 7, 2008; 105(40): 15541 - 15546. [Abstract] [Full Text] [PDF] N. Giuliani, G. Lisignoli, S. Colla, M. Lazzaretti, P. Storti, C. Mancini, S. Bonomini, C. Manferdini, K. Codeluppi, A. Facchini, et al. CC-Chemokine Ligand 20/Macrophage Inflammatory Protein-3{alpha} and CC-Chemokine Receptor 6 Are Overexpressed in Myeloma Microenvironment Related to Osteolytic Bone Lesions Cancer Res., August 15, 2008; 68(16): 6840 - 6850. [Abstract] [Full Text] [PDF] J. Lorenzo, M. Horowitz, and Y. Choi Osteoimmunology: Interactions of the Bone and Immune System Endocr. Rev., June 1, 2008; 29(4): 403 - 440. [Abstract] [Full Text] [PDF] G.E. Wise and G.J. King Mechanisms of Tooth Eruption and Orthodontic Tooth Movement Journal of Dental Research, May 1, 2008; 87(5): 414 - 434. [Abstract] [Full Text] [PDF] T.A. Silva, G.P. Garlet, S.Y. Fukada, J.S. Silva, and F.Q. Cunha Chemokines in Oral Inflammatory Diseases: Apical Periodontitis and Periodontal Disease Journal of Dental Research, April 1, 2007; 86(4): 306 - 319. [Abstract] [Full Text] [PDF] M. Yang, G. Mailhot, M. J. Birnbaum, C. A. MacKay, A. Mason-Savas, and P. R. Odgren Expression of and Role for Ovarian Cancer G-protein-coupled Receptor 1 (OGR1) during Osteoclastogenesis J. Biol. Chem., August 18, 2006; 281(33): 23598 - 23605. [Abstract] [Full Text] [PDF]

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