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Blood, 15 December 2006, Vol. 108, No. 13, pp. 3976-3978.
Prepublished online as a Blood First Edition Paper on August 22, 2006; DOI 10.1182/blood-2006-05-024075.
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PLENARY PAPERS
A transgenic zebrafish model of neutrophilic inflammation
Stephen A. Renshaw,
Catherine A. Loynes,
Daniel M.I. Trushell,
Stone Elworthy,
Philip W. Ingham, and
Moira K.B. Whyte
From the MRC Centre for Developmental and Biomedical Genetics, the Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, and the Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom.
We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.
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