Blood, 15 September 2006, Vol. 108, No. 6, pp. 1991-1998.
Prepublished online as a Blood First Edition Paper on May 25, 2006; DOI 10.1182/blood-2006-02-001354.
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NEOPLASIA
Dysregulated TCL1 requires the germinal center and genome instability for mature B-cell transformation
Rhine R. Shen,
David O. Ferguson,
Mathilde Renard,
Katrina K. Hoyer,
Unkyu Kim,
Xingpei Hao,
Frederick W. Alt,
Robert G. Roeder,
Herbert C. Morse, III, and
Michael A. Teitell
From the Departments of Pathology and Laboratory Medicine and Jonsson Comprehensive Cancer Center and Molecular Biology Institute, David Geffen School of Medicine at University of California at Los Angeles, CA; Howard Hughes Medical Institute and Children's Hospital, Center for Blood Research and Department of Genetics, Harvard Medical School, Boston, MA; Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY; and Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Rockville, MD.
Most lymphomas arise by transformation of germinal center (GC) B cells. TCL1, a proto-oncogene first recognized for its role in T-cell transformation, also induces GC B-cell malignancies when dysregulated in pEµ-B29-TCL1 transgenic (TCL1-tg) mice. Clonal B-cell lymphomas develop from polyclonal populations with latencies of 4 months or more, suggesting that secondary genetic events are required for full transformation. The goals of this study were to determine the GC-related effects of TCL1 dysregulation that contribute to tumor initiation and to identify companion genetic alterations in tumors that function in disease progression. We report that compared with wild-type (WT) cells, B cells from TCL1-tg mice activated in a manner resembling a T-dependent GC reaction show enhanced resistance to FAS-mediated apoptosis with CD40 stimulation, independent of a B-cell antigen receptor (BCR) rescue signal. Mitogenic stimulation of TCL1-tg B cells also resulted in increased expression of Aicda. These GC-related enhancements in survival and Aicda expression could underlie B-cell transformation. Supporting this notion, no B-cell lymphomas developed for 20 months when TCL1-tg mice were crossed onto an Oct coactivator from B cell (OCA-B)–deficient background to yield mice incapable of forming GCs. Spectral karyotype analyses showed that GC lymphomas from TCL1-tg mice exhibit recurrent chromosome translocations and trisomy 15, with corresponding MYC overexpression. We conclude that pEµ-B29-TCL1 transgenic B cells primed for transformation must experience the GC environment and, for at least some, develop genome instability to become fully malignant.
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