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 Blood, 15 September 2006, Vol. 108, No. 6, pp. 2121-2123.
Prepublished online as a Blood First Edition Paper on May 30, 2006; DOI 10.1182/blood-2006-03-013599.

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TRANSPLANTATION
Brief report

Short-term repopulating cells with myeloid potential in human mobilized peripheral blood do not have a side population (SP) phenotype

Marlene Fischer, Manfred Schmidt, Silke Klingenberg, Connie J. Eaves, Christof von Kalle, and Hanno Glimm

From the Department of Internal Medicine I, Albert-Ludwigs-University, Freiburg, Germany; the Faculty of Biology, Albert-Ludwigs-University, Freiburg, Germany; the Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University, Freiburg, Germany; the Terry Fox Laboratory, British Columbia Cancer Agency, University of British Columbia, Vancouver, Canada; the Department of Medical Genetics, University of British Columbia, Vancouver, Canada; the National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany; and the Experimental Hematology Research Foundation, Molecular and Gene Therapy Program, Cincinnati Children's Hospital, OH.

Clinical use of purified hematopoietic stem cells in myeloablated patients requires cotransplantation of short-term repopulating cells (STRCs) to ensure timely count recovery. Here, we investigated the flow fluorescence-based side population (SP) phenotype of mobilized human peripheral blood (mPB) cells that rapidly repopulate the highly permissive nonobese diabetic/severe combined immunodeficient (NOD/SCID)–beta2 microglobulin–/– mouse. No SP cells from this source regenerated detectable progeny in these mice before 8 weeks, although by 12 weeks human B-lymphoid cells were seen in some recipients of SP mPB cells. All myeloid reconstituting activity, including that seen within 3 weeks after transplantation, was associated with the non-SP fraction. Isolation of SP cells depletes human mPB of the rapid myeloid reconstitution capacity provided by myeloid-restricted STRCs which are vital for early hematologic recovery in clinical transplant recipients.


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