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Blood, 1 November 2006, Vol. 108, No. 9, pp. 3112-3120. Prepublished online as a Blood First Edition Paper on July 11, 2006; DOI 10.1182/blood-2006-04-016832. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-04-016832v1 108/9/3112    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Li, S. S. Articles by Sundqvist, K.-G. Search for Related Content PubMed PubMed Citation Articles by Li, S. S. Articles by Sundqvist, K.-G. Related Collections Immunobiology Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Endogenous thrombospondin-1 is a cell-surface ligand for regulation of integrin-dependent T-lymphocyte adhesion Shu Shun Li, Zhiwen Liu, Mehmet Uzunel, and Karl-Gösta Sundqvist From the Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska Institute at Karolinska University Hospital Huddinge, Stockholm, Sweden; and the Department of Clinical Microbiology, Division of Clinical Immunology, Umeå University, Umeå, Sweden. Lymphocyte adhesion to cells and extracellular matrix (ECM) via integrins plays a pivotal role for the function of the immune system. We show here that endogenous thrombospondin-1 (TSP-1) is a cell-surface ligand for cis interaction of surface receptors in T lymphocytes controlled by integrins and the T-cell antigen receptor (TCR/CD3). Stimulation of CD3 triggers rapid surface expression of TSP-1 in quiescent T cells, whereas activated cells express TSP-1 constitutively. Endogenous TSP-1 is attached to lipoprotein receptor-related protein 1 (LRP1/CD91) and calreticulin (CRT) on the cell surface through its NH2-terminal domain. Adhesion via integrins to ICAM-1 or ECM components up-regulates TSP turnover dramatically from a low level in nonadherent cells, whereas CD3 stimulation inhibits TSP turnover through interference with CD91/CRT-mediated internalization. Integrin-associated protein (IAP/CD47) is essential for TSP turnover and adhesion through interaction with the C-terminal domain of TSP-1 in response to triggering signals delivered at the NH2-terminal. These results indicate that endogenous TSP-1 connects separate cell-surface receptors functionally and regulates T-cell adhesion. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Z. Liu, M. Christensson, A. Forslow, I. De Meester, and K.-G. Sundqvist A CD26-Controlled Cell Surface Cascade for Regulation of T Cell Motility and Chemokine Signals J. Immunol., September 15, 2009; 183(6): 3616 - 3624. [Abstract] [Full Text] [PDF] S. Bouguermouh, V. Q. Van, J. Martel, P. Gautier, M. Rubio, and M. Sarfati CD47 Expression on T Cell Is a Self-Control Negative Regulator of Type 1 Immune Response J. Immunol., June 15, 2008; 180(12): 8073 - 8082. [Abstract] [Full Text] [PDF]

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