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Blood, 1 June 2007, Vol. 109, No. 11, pp. 4806-4809. Prepublished online as a Blood First Edition Paper on February 20, 2007; DOI 10.1182/blood-2006-09-047449. This Article Full Text Full Text (PDF) Supplemental Methods, Table, and Figures All Versions of this Article: blood-2006-09-047449v1 109/11/4806    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Newland, S. A. Articles by Campbell, D. R. Search for Related Content PubMed PubMed Citation Articles by Newland, S. A. Articles by Campbell, D. R. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Signal Transduction Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Brief Report The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro Stephen A. Newland1, Iain C. Macaulay2, Andres R. Floto1, Edwin C. de Vet3, Willem H. Ouwehand2, Nicholas A. Watkins2, Paul A. Lyons1, and Duncan R. Campbell4 1 Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom; 2 National Blood Service Cambridge and Department of Haematology, University of Cambridge, Cambridge, United Kingdom; 3 MRC Rosalind Franklin Centre for Genomics Research, Hinxton, Cambridge, United Kingdom; 4 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom The G6B cell-surface receptor, which contains a single Ig-like domain, has been shown to bind to SHP-1 and SHP-2 after phosphorylation of 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail, classifying this protein as a new member of the family of inhibitory receptors. In this study, we demonstrate by real-time polymerase chain reaction (PCR) and Western-blot analysis that G6B is expressed on platelets. Cross-linking of G6B with polyclonal antisera has a significant inhibitory effect on platelet aggregation and activation by agonists such as ADP and collagen-related peptide (CRP). This inhibitory function of G6B appears to operate in a calcium-independent manner. Our results suggest that G6B represents a novel inhibitory receptor found on the surface of platelets and that it could be an antithrombotic drug target. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. C. Macaulay, M. R. Tijssen, D. C. Thijssen-Timmer, A. Gusnanto, M. Steward, P. Burns, C. F. Langford, P. D. Ellis, F. Dudbridge, J.-J. Zwaginga, et al. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins Blood, April 15, 2007; 109(8): 3260 - 3269. [Abstract] [Full Text] [PDF]

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