SEARCH:   Advanced

Blood, 1 June 2007, Vol. 109, No. 11, pp. 4890-4898. Prepublished online as a Blood First Edition Paper on February 15, 2007; DOI 10.1182/blood-2006-05-022277. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-05-022277v1 109/11/4890    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Garrido, P. Articles by Orfao, A. Search for Related Content PubMed PubMed Citation Articles by Garrido, P. Articles by Orfao, A. Related Collections Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Monoclonal TCR-Vβ13.1+/CD4+/NKa+/CD8–/+dim T-LGL lymphocytosis: evidence for an antigen-driven chronic T-cell stimulation origin Pilar Garrido1, Francisco Ruiz-Cabello2, Paloma Bárcena3,4, Yorick Sandberg5, Julia Cantón1, Margarida Lima6, Ana Balanzategui4,7, Marcos González4,7, Miguel Angel López-Nevot1, Anton W. Langerak5, Andrés C. García-Montero3,4, Julia Almeida3,4, and Alberto Orfao3,4 1 Servicio de Hematología, Hospital Universitario Virgen de las Nieves, Granada, Spain; 2 Servicio de Análisis Clínicos, Hospital Universitario Virgen de las Nieves, Granada, Spain; 3 Servicio de Citometría & Departamento de Medicina, Universidad de Salamanca (USAL), Spain; 4 Instituto de Biología Molecular y Celular del Cáncer, Centro de Investigación del Cáncer/IBMCC (CSIC-USAL), Salamanca, Spain; 5 Department of Immunology, Erasmus Medical Center, Rotterdam, The Netherlands; 6 Servicio de Hematología, Hospital Geral de Santo António, Porto, Portugal; 7 Servicio de Hematología, Hospital Universitario de Salamanca, Spain Monoclonal TCRβ+/CD4+ T-large granular lymphocyte (T-LGL) lymphocytosis is a T-cell disorder with a restricted TCR-Vβ repertoire. In the present study we explored the potential association between the expanded TCR-Vβ families, the CDR3 sequences of the TCR-Vβ gene, and the HLA genotype of patients with monoclonal TCRβ+/CD4+ T-LGL lymphocytosis. For that purpose, 36 patients with monoclonal TCRβ+/CD4+ T-LGL lymphocytosis (15 TCR-Vβ13.1 versus 21 non–TCR-Vβ13.1) were selected. For each patient, both the HLA (class I and II) genotype and the DNA sequences of the VDJ-rearranged TCR-Vβ were analyzed. Our results show a clear association between the TCR-Vβ repertoire and the HLA genotype, all TCR-Vβ13.1+ cases being HLA-DRB1*0701 (P = .004). Interestingly, the HLA-DR7/TCR-Vβ13.1–restricted T-cell expansions displayed a highly homogeneous and strikingly similar TCR arising from the use of common TCR-Vβ gene segments, which shared (1) unique CDR3 structural features with a constantly short length, (2) similar combinatorial gene rearrangements with frequent usage of the Jβ1.1 gene, and (3) a homolog consensus protein sequence at recombination junctions. Overall, these findings strongly support the existence of a common antigen-driven origin for monoclonal CD4+ T-LGL lymphocytosis, with the identification of the exact peptides presented to the expanded T cells deserving further investigations. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. J. M. ten Berge and R. A. W. van Lier Attack of the CD4 clones Blood, February 15, 2008; 111(4): 1750 - 1751. [Full Text] [PDF] L. Crompton, N. Khan, R. Khanna, L. Nayak, and P. A. H. Moss CD4+ T cells specific for glycoprotein B from cytomegalovirus exhibit extreme conservation of T-cell receptor usage between different individuals Blood, February 15, 2008; 111(4): 2053 - 2061. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020