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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5242-5250. Prepublished online as a Blood First Edition Paper on March 8, 2007; DOI 10.1182/blood-2006-06-030619. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-06-030619v1 109/12/5242    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Panes, O. Articles by Mezzano, D. Search for Related Content PubMed PubMed Citation Articles by Panes, O. Articles by Mezzano, D. Related Collections Hemostasis, Thrombosis, and Vascular Biology Gene Expression Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Human platelets synthesize and express functional tissue factor Olga Panes1, Valeria Matus1, Claudia G. Sáez1, Teresa Quiroga2, Jaime Pereira1, and Diego Mezzano1 1 Department of Hematology-Oncology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile; 2 Clinical Laboratory, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile The source and significance of bloodborne tissue factor (TF) are controversial. TF mRNA, protein, and TF-dependent procoagulant activity (PCA) have been detected in human platelets, but direct evidence of TF synthesis is missing. Nonstimulated monocyte-free platelets from most patients expressed TF mRNA, which was enhanced or induced in all of them after platelet activation. Immunoprecipitation assays revealed TF protein (mainly of a molecular weight [Mr] of approximately 47 kDa, with other bands of approximately 35 and approximately 60 kDa) in nonstimulated platelet membranes, which also increased after activation. This enhancement was concomitant with TF translocation to the plasma membrane, as demonstrated by immunofluorescence–confocal microscopy and biotinylation of membrane proteins. Platelet PCA, assessed by factor Xa (FXa) generation, was induced after activation and was inhibited by 48% and 76% with anti-TF and anti-FVIIa, respectively, but not by intrinsic pathway inhibitors. Platelets incorporated [35S]-methionine into TF proteins with Mr of approximately 47 kDa, approximately 35 kDa, and approximately 60 kDa, more intensely after activation. Puromycin but not actinomycin D or DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) inhibited TF neosynthesis. Thus, human platelets not only assemble the clotting reactions on their membrane, but also supply their own TF for thrombin generation in a timely and spatially circumscribed process. These observations simplify, unify, and provide a more coherent formulation of the current cell-based model of hemostasis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Platelet tissue factor comes of age Andrew S. Weyrich, Hansjörg Schwertz, and Nigel Mackman Blood 2007 109: 5069-5070. [Full Text] [PDF] This article has been cited by other articles: N. Mackman and M. Taubman Tissue Factor: Past, Present, and Future Arterioscler Thromb Vasc Biol, December 1, 2009; 29(12): 1986 - 1988. [Full Text] [PDF] S. Butenas, T. Orfeo, and K. G. Mann Tissue Factor in Coagulation: Which? Where? When? 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