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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5337-5345. Prepublished online as a Blood First Edition Paper on March 5, 2007; DOI 10.1182/blood-2006-09-048058. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-09-048058v1 109/12/5337    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dominguez-Soto, A. Articles by Corbi, A. L. Search for Related Content PubMed PubMed Citation Articles by Dominguez-Soto, A. Articles by Corbi, A. L. Related Collections Immunobiology Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY The DC-SIGN–related lectin LSECtin mediates antigen capture and pathogen binding by human myeloid cells Angeles Dominguez-Soto1, Laura Aragoneses-Fenoll1, Enrique Martin-Gayo2, Lorena Martinez-Prats3, Maria Colmenares1, Marisa Naranjo-Gomez4, Francesc E. Borras4, Pilar Munoz5, Mercedes Zubiaur5, Maria L. Toribio2, Rafael Delgado3, and Angel L. Corbi1 1 Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid; 2 Centro de Biología Molecular "Severo Ochoa," Consejo Superior de Investigaciones Científicas, Madrid; 3 Hospital Doce de Octubre, Madrid; 4 Hospital Universitari Germans Trias i Pujol, Badalona; 5 Instituto de Parasitología y Biomedicina, Consejo Superior de Investigaciones Científicas, Granada, Spain Liver and lymph node sinusoidal endothelial cell C-type lectin (LSECtin [CLEC4G]) is a C-type lectin encoded within the liver/lymph node–specific intercellular adhesion molecule-3–grabbing nonintegrin (L-SIGN)/dendritic cell–specific intercellular adhesion molecule-3–grabbing nonintegrin (DC-SIGN)/CD23 gene cluster. LSECtin expression has been previously described as restricted to sinusoidal endothelial cells of the liver and lymph node. We now report LSECtin expression in human peripheral blood and thymic dendritic cells isolated ex vivo. LSECtin is also detected in monocyte-derived macrophages and dendritic cells at the RNA and protein level. In vitro, interleukin-4 (IL-4) induces the expression of 3 LSECtin alternatively spliced isoforms, including a potentially soluble form (2 isoform) and a shorter version of the prototypic molecule (3/4 isoform). LSECtin functions as a pathogen receptor, because its expression confers Ebola virus–binding capacity to leukemic cells. Sugar-binding studies indicate that LSECtin specifically recognizes N-acetyl-glucosamine, whereas no LSECtin binding to Mannan- or N-acetyl-galactosamine–containing matrices are observed. Antibody or ligand-mediated engagement triggers a rapid internalization of LSECtin,which is dependent on tyrosine and diglutamic-containing motifs within the cytoplasmic tail. Therefore, LSECtin is a pathogen-associated molecular pattern receptor in human myeloid cells. In addition, our results suggest that LSECtin participates in antigen uptake and internalization, and might be a suitable target molecule in vaccination strategies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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