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Blood, 15 February 2007, Vol. 109, No. 4, pp. 1433-1441. Prepublished online as a Blood First Edition Paper on October 24, 2006; DOI 10.1182/blood-2006-06-031898. This Article Full Text Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-06-031898v1 109/4/1433    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tober, J. Articles by Palis, J. Search for Related Content PubMed PubMed Citation Articles by Tober, J. Articles by Palis, J. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS The megakaryocyte lineage originates from hemangioblast precursors and is an integral component both of primitive and of definitive hematopoiesis Joanna Tober1, Anne Koniski1, Kathleen E. McGrath1,2, Radhika Vemishetti3, Rachael Emerson1, Karen K. L. de Mesy-Bentley4, Richard Waugh3, and James Palis1,2 1 Center for Pediatric Biomedical Research, 2 Department of Pediatrics, 3 Department of Biomedical Engineering, and 4 Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, NY In the adult, platelets are derived from unipotential megakaryocyte colony-forming cells (Meg-CFCs) that arise from bipotential megakaryocyte/erythroid progenitors (MEPs). To better define the developmental origin of the megakaryocyte lineage, several aspects of megakaryopoiesis, including progenitors, maturing megakaryocytes, and circulating platelets, were examined in the murine embryo. We found that a majority of hemangioblast precursors during early gastrulation contains megakaryocyte potential. Combining progenitor assays with immunohistochemical analysis, we identified 2 waves of MEPs in the yolk sac associated with the primitive and definitive erythroid lineages. Primitive MEPs emerge at E7.25 along with megakaryocyte and primitive erythroid progenitors, indicating that primitive hematopoiesis is bilineage in nature. Subsequently, definitive MEPs expand in the yolk sac with Meg-CFCs and definitive erythroid progenitors. The first GP1bß-positive cells in the conceptus were identified in the yolk sac at E9.5, while large, highly reticulated platelets were detected in the embryonic bloodstream beginning at E10.5. At this time, the number of megakaryocyte progenitors begins to decline in the yolk sac and expand in the fetal liver. We conclude that the megakaryocyte lineage initially originates from hemangioblast precursors during early gastrulation and is closely associated both with primitive and with definitive erythroid lineages in the yolk sac prior to the transition of hematopoiesis to intraembryonic sites. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: On the origins of megakaryocytes John D. Crispino Blood 2007 109: 1340-1341. [Full Text] [PDF] This article has been cited by other articles: R. Shimizu, E. Kobayashi, J. D. Engel, and M. Yamamoto Induction of hyperproliferative fetal megakaryopoiesis by an N-terminally truncated GATA1 mutant Genes Cells, September 1, 2009; 14(9): 1119 - 1131. [Abstract] [Full Text] [PDF] P. Gadue and M. J. 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Phipps 15-deoxy-{Delta}12,14-PGJ2 enhances platelet production from megakaryocytes Blood, November 15, 2008; 112(10): 4051 - 4060. [Abstract] [Full Text] [PDF] H. Nishikii, K. Eto, N. Tamura, K. Hattori, B. Heissig, T. Kanaji, A. Sawaguchi, S. Goto, J. Ware, and H. Nakauchi Metalloproteinase regulation improves in vitro generation of efficacious platelets from mouse embryonic stem cells J. Exp. Med., August 4, 2008; 205(8): 1917 - 1927. [Abstract] [Full Text] [PDF] J. Tober, K. E. McGrath, and J. Palis Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-myb Blood, March 1, 2008; 111(5): 2636 - 2639. [Abstract] [Full Text] [PDF] J. A. Martinez-Agosto, H. K.A. Mikkola, V. Hartenstein, and U. Banerjee The hematopoietic stem cell and its niche: a comparative view Genes & Dev., December 1, 2007; 21(23): 3044 - 3060. [Abstract] [Full Text] [PDF]

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