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Blood, 15 February 2007, Vol. 109, No. 4, pp. 1503-1506. Prepublished online as a Blood First Edition Paper on October 10, 2006; DOI 10.1182/blood-2006-04-020362. This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2006-04-020362v1 109/4/1503    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tiedt, R. Articles by Skoda, R. C. Search for Related Content PubMed PubMed Citation Articles by Tiedt, R. Articles by Skoda, R. C. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Gene Expression Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Pf4-Cre transgenic mice allow the generation of lineage-restricted gene knockouts for studying megakaryocyte and platelet function in vivo Ralph Tiedt1, Tibor Schomber1, Hui Hao-Shen1, and Radek C. Skoda1 1 Department of Research and Experimental Hematology, University Hospital Basel, Switzerland To generate transgenic mice that express Cre-recombinase exclusively in the megakaryocytic lineage, we modified a mouse bacterial artificial chromosome (BAC) clone by homologous recombination and replaced the first exon of the platelet factor 4 (Pf4), also called CXCL4, with a codon-improved Cre cDNA. Several strains expressing the transgene were obtained and one strain, Q3, was studied in detail. Crossing Q3 mice with the ROSA26-lacZ reporter strain showed that Cre-recombinase activity was confined to megakaryocytes. These results were further verified by crossing the Q3 mice with a strain containing loxP-flanked integrin ß1. Excision of this conditional allele in megakaryocytes was complete at the DNA level, and platelets were virtually devoid of the integrin ß1 protein. The Pf4-Cre transgenic strain will be a valuable tool to study megakaryopoiesis, platelet formation, and platelet function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Happy to have the megakaryocyte blues Andrew D. Leavitt Blood 2007 109: 1341. [Full Text] [PDF] This article has been cited by other articles: M. Foller, S. Feil, K. Ghoreschi, S. Koka, A. Gerling, M. Thunemann, F. Hofmann, B. Schuler, J. Vogel, B. Pichler, et al. Anemia and splenomegaly in cGKI-deficient mice PNAS, May 6, 2008; 105(18): 6771 - 6776. [Abstract] [Full Text] [PDF] J. E. Italiano Jr Megakaryocyte and platelet biology: getting your FAKs straight Blood, January 15, 2008; 111(2): 482 - 483. [Full Text] [PDF] I. S. Hitchcock, N. E. Fox, N. Prevost, K. Sear, S. J. Shattil, and K. Kaushansky Roles of focal adhesion kinase (FAK) in megakaryopoiesis and platelet function: studies using a megakaryocyte lineage specific FAK knockout Blood, January 15, 2008; 111(2): 596 - 604. [Abstract] [Full Text] [PDF] B. G. Petrich, P. Marchese, Z. M. Ruggeri, S. Spiess, R. A.M. Weichert, F. Ye, R. Tiedt, R. C. Skoda, S. J. Monkley, D. R. Critchley, et al. Talin is required for integrin-mediated platelet function in hemostasis and thrombosis J. Exp. Med., December 24, 2007; 204(13): 3103 - 3111. [Abstract] [Full Text] [PDF] C. Leon, A. Eckly, B. Hechler, B. Aleil, M. Freund, C. Ravanat, M. Jourdain, C. Nonne, J. Weber, R. Tiedt, et al. Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion Blood, November 1, 2007; 110(9): 3183 - 3191. [Abstract] [Full Text] [PDF]

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