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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2346-2355. Prepublished online as a Blood First Edition Paper on November 21, 2006; DOI 10.1182/blood-2006-04-019034. This Article Full Text Full Text (PDF) Supplemental Methods and Table Supplemental Figures Supplemental Figures All Versions of this Article: blood-2006-04-019034v1 109/6/2346    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dorn, T. Articles by Brakebusch, C. Search for Related Content PubMed PubMed Citation Articles by Dorn, T. Articles by Brakebusch, C. Related Collections Hematopoiesis and Stem Cells Immunobiology Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS RhoH is important for positive thymocyte selection and T-cell receptor signaling Tatjana Dorn1,2, Ursula Kuhn1,3, Gerd Bungartz1,3, Sebastian Stiller1, Martina Bauer1,3, Joachim Ellwart4, Thorsten Peters5, Karin Scharffetter-Kochanek5, Monika Semmrich6, Melanie Laschinger6, Bernhard Holzmann6, Wolfgang E. F. Klinkert7, Per Thor Straten8, Tania Køllgaard8, Michael Sixt3, and Cord Brakebusch1,2 1 Max Planck Institute of Biochemistry, Heisenberg Group Regulation of Cytoskeletal Organization, Martinsried, Germany; 2 Department of Molecular Pathology, University of Copenhagen, Denmark; 3 Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany; 4 GSF, Institute of Molecular Immunology, München, Germany; 5 University of Ulm, Clinic for Dermatology and Allergology, Germany; 6 Department of Surgery, Technische Universität München, Germany; 7 Department of Neuroimmunology, Max Planck Institute of Neurobiology, Martinsried, Germany; 8 Department of Hematology, Center for Cancer Immunotherapy, University Hospital Herlev, Denmark RhoH is a small GTPase expressed only in the hematopoietic system. With the use of mice with targeted disruption of the RhoH gene, we demonstrated that RhoH is crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Furthermore, the differentiation and expansion of DN3 and DN4 thymocytes in vitro were severely impaired. These defects corresponded to defective TCR signaling. Although RhoH is not required for TCR-induced activation of ZAP70 and ZAP70-mediated activation of p38, it is crucial for the tyrosine phosphorylation of LAT, PLC1, and Vav1 and for the activation of Erk and calcium influx. These data suggest that RhoH is important for pre–TCR and TCR signaling because it allows the efficient interaction of ZAP70 with the LAT signalosome, thus regulating thymocyte development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M.-H. Fortier, E. Caron, M.-P. Hardy, G. Voisin, S. Lemieux, C. Perreault, and P. Thibault The MHC class I peptide repertoire is molded by the transcriptome J. Exp. Med., March 17, 2008; 205(3): 595 - 610. [Abstract] [Full Text] [PDF]

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