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 Blood, 15 March 2007, Vol. 109, No. 6, pp. 2356-2364.
Prepublished online as a Blood First Edition Paper on November 9, 2006; DOI 10.1182/blood-2006-06-030031.

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HEMATOPOIESIS

Gfi1b:green fluorescent protein knock-in mice reveal a dynamic expression pattern of Gfi1b during hematopoiesis that is largely complementary to Gfi1

Lothar Vassen1, Taro Okayama1,2, and Tarik Möröy1,2

1 Institut für Zellbiologie (Tumorforschung), Universitätsklinikum Essen, Germany; 2 Institut de recherches cliniques de Montreal, Department de microbiologie et immunologie, Université de Montréal, QC, Canada

Gfi1b and Gfi1 are 37- and 55-kDa transcriptional repressors that share common features such as a 20-amino acid (aa) N-terminal SNAG domain, a nonconserved intermediary domain, and 6 highly conserved C-terminal zinc fingers. Both gene loci are under autoregulatory and cross-regulatory feedback control. We have generated a reporter mouse strain by inserting the cDNA for green fluorescent protein (GFP) into the Gfi1b gene locus which allowed us to follow Gfi1b expression during hematopoiesis and lymphopoiesis by measuring green fluorescence. We found highly dynamic expression patterns of Gfi1b in erythroid cells, megakaryocytes, and their progenitor cells (MEPS) where Gfi1 is not detected. Vice versa, Gfi1b could not be found in granulocytes, activated macrophages, or their granulomonocytic precursors (GMPs) or in mature naive or activated lymphocytes where Gfi1 is expressed, suggesting a complementary regulation of both loci during hematopoiesis. However, Gfi1b was found to be up-regulated in early stages of B-cell and in a subset of early T-cell development, where Gfi1 is also present, suggesting that cross-regulation of both loci exists but is cell-type specific.


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