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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2406-2415. Prepublished online as a Blood First Edition Paper on November 7, 2006; DOI 10.1182/blood-2006-06-031039. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-06-031039v1 109/6/2406    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shinoda, G. Articles by Nakahata, T. Search for Related Content PubMed PubMed Citation Articles by Shinoda, G. Articles by Nakahata, T. Related Collections Cell Adhesion and Motility Stem Cells in Hematology Hematopoiesis Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS 4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells Gen Shinoda1, Katsutsugu Umeda1, Toshio Heike1, Masato Arai1, Akira Niwa1, Feng Ma1, Hirofumi Suemori2, Hong Yuan Luo3, David H. K. Chui3, Ryuzo Torii4, Masabumi Shibuya5, Norio Nakatsuji6, and Tatsutoshi Nakahata1 1 Department of Pediatrics, Graduate School of Medicine, 2 Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, and 6 Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Japan; 3 Department of Medicine, Boston University School of Medicine, MA; 4 Research Center for Animal Life Science, Shiga University of Medical Science, Japan; 5 Division of Genetics, Institute of Medical Science, University of Tokyo, Japan The mechanism of commencement of hematopoiesis in blood islands of the yolk sac and the aorta-gonad-mesonephros (AGM) region during primate embryogenesis remains elusive. In this study, we demonstrated that VE-cadherin+CD45– endothelial cells derived from nonhuman primate embryonic stem cells are able to generate primitive and definitive hematopoietic cells sequentially, as revealed by immunostaining of floating erythrocytes and colony-forming assay in cultures. Single bipotential progenitors for hematopoietic and endothelial lineages are included in this endothelial cell population. Furthermore, hemogenic activity of these endothelial cells is observed exclusively in the 4-integrin+ subpopulation; bipotential progenitors are 4-fold enriched in this subpopulation. The kinetics of this hemogenic subpopulation is similar to that of hemogenic endothelial cells previously reported in the yolk sac and the AGM region in vivo in that they emerge for only a limited time. We suggest that VE-cadherin+CD45–4-integrin+ endothelial cells are involved in primitive and definitive hematopoiesis during primate embryogenesis, though VE-cadherin–CD45–4-integrin+ cells are the primary sources for primitive hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Wianny, A. Bernat, C. Huissoud, G. Marcy, S. Markossian, V. Cortay, P. Giroud, V. Leviel, H. Kennedy, P. Savatier, et al. Derivation and Cloning of a Novel Rhesus Embryonic Stem Cell Line Stably Expressing Tau-Green Fluorescent Protein Stem Cells, June 1, 2008; 26(6): 1444 - 1453. [Abstract] [Full Text] [PDF]

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