SEARCH:   Advanced

Blood, 1 May 2007, Vol. 109, No. 9, pp. 3706-3712. Prepublished online as a Blood First Edition Paper on January 16, 2007; DOI 10.1182/blood-2006-08-041384. This Article Full Text Full Text (PDF) Supplemental Figure Erratum (v110,p17) All Versions of this Article: blood-2006-08-041384v1 109/9/3706    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhu, J. Articles by Emerson, S. G. Search for Related Content PubMed PubMed Citation Articles by Zhu, J. Articles by Emerson, S. G. Related Collections Hematopoiesis and Stem Cells Immunobiology Stem Cells in Hematology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells Jiang Zhu1, Russell Garrett1, Younghun Jung2, Yi Zhang1, Nacksung Kim3, Jingcheng Wang2, Gerard J. Joe1, Elizabeth Hexner1, Yongwon Choi1,3, Russell S. Taichman2, and Stephen G. Emerson1,3 1 Departments of Medicine, Pathology, and Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA; 2 Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI; 3 Abramson Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, PA Early B lymphopoiesis in mammals is induced within the bone marrow (BM) microenvironment, but which cells constitute this niche is not known. Previous studies had shown that osteoblasts (OBs) support hematopoietic stem cell (HSC) proliferation and myeloid differentiation. We now find that purified primary murine OBs also support the differentiation of primitive hematopoietic stem cells through lymphoid commitment and subsequent differentiation to all stages of B-cell precursors and mature B cells. Lin–Sca-1+Rag-2– BM cell differentiation to B cells requires their attachment to OBs in vitro, and this developmental process is mediated via VCAM-1, SDF-1, and IL-7 signaling induced by parathyroid hormone (PTH). Addition of cytokines produced by nonosteoblastic stromal cells (c-Kit ligand, IL-6, and IL-3) shifted the cultures toward myelopoiesis. Confirming the role of OBs in B lymphopoiesis, we found that selective elimination of osteoblasts in Col2.3-TK transgenic mice severely depleted pre-pro-B and pro-B cells from BM, preceding any decline in HSCs. Taken together, these results demonstrate that osteoblasts are both necessary and sufficient for murine B-cell commitment and maturation, and thereby constitute the cellular homolog of the avian bursa of Fabricius. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Chandra, E. Villanueva, E. Feketova, G. W. Machiedo, G. Hasko, E. A. Deitch, and Z. Spolarics Endotoxemia down-regulates bone marrow lymphopoiesis but stimulates myelopoiesis: the effect of G6PD deficiency J. Leukoc. Biol., June 1, 2008; 83(6): 1541 - 1550. [Abstract] [Full Text] [PDF] S. Lymperi, N. Horwood, S. Marley, M. Y. Gordon, A. P. Cope, and F. Dazzi Strontium can increase some osteoblasts without increasing hematopoietic stem cells Blood, February 1, 2008; 111(3): 1173 - 1181. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020