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Blood, 1 July 2007, Vol. 110, No. 1, pp. 82-90. Prepublished online as a Blood First Edition Paper on March 14, 2007; DOI 10.1182/blood-2006-05-021352. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-05-021352v1 110/1/82    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jung, Y. Articles by Taichman, R. S. Search for Related Content PubMed PubMed Citation Articles by Jung, Y. Articles by Taichman, R. S. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Stem Cells in Hematology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation Younghun Jung1, Jingcheng Wang1, Junhui Song2, Yusuke Shiozawa1, Jianhua Wang1, Aaron Havens1, Zhuo Wang2, Yan-Xi Sun1, Stephen G. Emerson3, Paul H. Krebsbach2, and Russell S. Taichman1 1 Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor; 2 Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor; 3 Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia and Haverford College, Haverford, PA Differentiation of hematopoietic stem cells (HSCs) after birth is largely restricted to the bone marrow cavity, where HSCs are associated closely with osteoblasts (OBs). How OBs localize HSCs to the endosteal niche remains unclear. To explore adhesive interactions between HSCs and OBs, a cell blot analysis was used that revealed 2 major bands that corresponded to monomers and multimers of annexin II (Anxa2). Immunohistochemistry revealed that OBs and marrow endothelial cells express Anxa2 at high levels. Function-blocking studies confirmed that Anxa2 mediates HSC adhesion mainly via the N-terminal portion of the Anxa2 peptide. Adhesion of HSCs to OBs derived from Anxa2-deficient animals (Anxa2–/–) was significantly impaired compared with OBs obtained from wild-type animals (Anxa2+/+). Moreover, fewer HSCs were found in the marrow of Anxa2–/– versus Anxa2+/+ animals. Short-term lodging, engraftment, and survival of irradiated mice with whole marrow cells were substantially inhibited by N-terminal peptide fragments of Anxa2 or anti-Anxa2 antibodies. Similar findings were noted in long-term competitive repopulation studies. Collectively, these findings reveal that Anxa2 regulates HSC homing and binding to the bone marrow microenvironment and suggest that Anxa2 is crucial for determining the bone marrow niche of HSCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. M. Drake, C. M. Stock, J. K. Nathan, P. Gip, K. P. K. Golden, B. Weinhold, R. Gerardy-Schahn, and C. R. Bertozzi Polysialic acid governs T-cell development by regulating progenitor access to the thymus PNAS, July 21, 2009; 106(29): 11995 - 12000. [Abstract] [Full Text] [PDF] R. E. Coleman, T. A. Guise, A. Lipton, G. D. Roodman, J. R. Berenson, J.-J. Body, B. F. Boyce, L. M. Calvi, P. Hadji, E. V. McCloskey, et al. Advancing Treatment for Metastatic Bone Cancer: Consensus Recommendations from the Second Cambridge Conference Clin. Cancer Res., October 15, 2008; 14(20): 6387 - 6395. [Abstract] [Full Text] [PDF] J. Lorenzo, M. Horowitz, and Y. Choi Osteoimmunology: Interactions of the Bone and Immune System Endocr. Rev., June 1, 2008; 29(4): 403 - 440. [Abstract] [Full Text] [PDF]

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