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Blood, 15 August 2007, Vol. 110, No. 4, pp. 1359-1361. Prepublished online as a Blood First Edition Paper on April 24, 2007; DOI 10.1182/blood-2007-03-079848. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-03-079848v1 110/4/1359    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kjaersgaard, M. Articles by Semple, J. W. Search for Related Content PubMed PubMed Citation Articles by Kjaersgaard, M. Articles by Semple, J. W. Related Collections Hemostasis, Thrombosis, and Vascular Biology Phagocytes Transfusion Medicine Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE Brief Report Epitope specificity and isotype of monoclonal anti-D antibodies dictate their ability to inhibit phagocytosis of opsonized platelets Mimi Kjaersgaard1, Rukhsana Aslam2,3, Michael Kim3, Edwin R. Speck2,3, John Freedman2,6, Donald I. H. Stewart7, Erik J. Wiersma7, and John W. Semple2,6,8 1 Department of Pediatrics, Aarhus University Hospital, Skejby, Denmark; 2 Canadian Blood Services, Ottawa, ON; 3 The Toronto Platelet Immunobiology Group, ON, Canada; 4 Department of Laboratory Medicine, St. Michael's Hospital, Toronto, ON, Canada; 5 Department of Medicine 6 Department of Laboratory Medicine and Pathobiology, University of Toronto, ON, Canada; 7 Department of Research, Cangene, Mississauga, ON, Canada; and 8 Department of Pharmacology, University of Toronto, ON, Canada Rh immune globulin (WinRho SDF; Cangene, Mississauga, ON, Canada) is an effective treatment for autoimmune thrombocytopenic purpura; however, maintaining a sustained supply for its use in autoimmune thrombocytopenic purpura and its primary indication, hemolytic disease of the newborn, makes the development of alternative reagents desirable. We compared Rh immune globulin and 6 human monoclonal anti-D antibodies (MoAnti-D) with differing isotypes and specificities for their ability to opsonize erythrocytes and inhibit platelet phagocytosis in an in vitro assay. Results demonstrated that opsonization of erythrocytes with Rh immune globulin significantly (P < .001) reduced phagocytosis of fluorescently labeled opsonized platelets in an Fc-dependent manner. Of the MoAnti-D that shared specificity but differed in isotype, only IgG3 antibodies could significantly (P < .001) inhibit platelet phagocytosis. In contrast, 2 MoAnti-D shared isotypes and differed in specificity; however, only one could significantly (P < .001) inhibit platelet phagocytosis. The results suggest that MoAnti-D epitope specificity and isotypes are critical requirements for optimal inhibition of opsonized platelet phagocytosis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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