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Blood, 1 September 2007, Vol. 110, No. 5, pp. 1492-1501. Prepublished online as a Blood First Edition Paper on May 7, 2007; DOI 10.1182/blood-2006-11-055772. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-11-055772v1 110/5/1492    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Flaumenhaft, R. Articles by Dvorak, A. M. Search for Related Content PubMed PubMed Citation Articles by Flaumenhaft, R. Articles by Dvorak, A. M. Related Collections Hemostasis, Thrombosis, and Vascular Biology Related Articles in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet plasma membrane Robert Flaumenhaft1, Nataliya Rozenvayn1, Dian Feng2, and Ann M. Dvorak2 Departments of 1 Medicine and 2 Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA SNARE proteins direct membrane fusion events required for platelet granule secretion. These proteins are oriented in cell membranes such that most of the protein resides in a cytosolic compartment. Evaluation of SNARE protein localization in activated platelets using immunonanogold staining and electron microscopy, however, demonstrated expression of SNAP-23 and syntaxin-2 on the extracellular surface of the platelet plasma membrane. Flow cytometry of intact platelets confirmed trypsin-sensitive SNAP-23 and syntaxin-2 localization to the extracellular surface of the plasma membrane. Acyl-protein thioesterase 1 and botulinum toxin C light chain released SNAP-23 and syntaxin-2, respectively, from the surface of intact platelets. When resting platelets were incubated with both acyl-protein thioesterase 1 and botulinum toxin C light chain, a complex that included both SNAP-23 and syntaxin-2 was detected in supernatants, indicating that extracellular SNARE proteins retain their ability to bind one another. These observations represent the first description of SNARE proteins on the extracellular surface of a cell. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Articles in Blood Online: Extracellular localization of epimorphin/syntaxin-2 Yohei Hirai, Mina J. Bissell, and Derek C. Radisky Blood 2007 110: 3082. [Full Text] [PDF] Response: Extracellular localization of platelet SNARE proteins Robert Flaumenhaft, Nataliya Rozenvayn, Dian Feng, and Ann M. Dvorak Blood 2007 110: 3082-3083. [Full Text] [PDF] This article has been cited by other articles: Y. Hirai, M. J. Bissell, and D. C. Radisky Extracellular localization of epimorphin/syntaxin-2 Blood, October 15, 2007; 110(8): 3082 - 3082. [Full Text] [PDF] R. Flaumenhaft, N. Rozenvayn, D. Feng, and A. M. Dvorak Response: Extracellular localization of platelet SNARE proteins Blood, October 15, 2007; 110(8): 3082 - 3083. [Full Text] [PDF]

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