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Blood, 15 September 2007, Vol. 110, No. 6, pp. 1950-1959. Prepublished online as a Blood First Edition Paper on May 25, 2007; DOI 10.1182/blood-2007-01-070003. This Article Full Text Full Text (PDF) Supplemental Methods and Figure All Versions of this Article: blood-2007-01-070003v1 110/6/1950    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by White, A. Articles by Caamaño, J. H. Search for Related Content PubMed PubMed Citation Articles by White, A. Articles by Caamaño, J. H. Related Collections Hematopoiesis and Stem Cells Immunobiology Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Lymphotoxin a-dependent and -independent signals regulate stromal organizer cell homeostasis during lymph node organogenesis Andrea White1, Damian Carragher1, Sonia Parnell1, Aichi Msaki2, Neil Perkins2, Peter Lane1, Eric Jenkinson1, Graham Anderson1, and Jorge H. Caamaño1 1 Division of Immunity and Infection, Institute for BioMedical Research-Medical Research Council (IBR-MRC) Centre for Immune Regulation, University of Birmingham Medical School, Birmingham; 2 Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom Lymph nodes provide specialized stromal microenvironments that support the recruitment and organization of T cells and B cells, enabling them to effectively participate in immune responses. While CD4+3– lymphoid tissue inducer cells (LTic's) are known to play a key role in influencing lymph node (LN) development, the mechanisms that regulate the development of stromal organizer cells are unclear. Here, we define an ontogenetic program of lymph node stromal cell maturation in relation to the requirement for LTic's. We also describe a lymph node reaggregation assay to study cell-cell interactions and lymphocyte recruitment to these organs that reproduces the in vivo events during lymph node development. In addition, analysis of the lymph node anlagen in normal and lymphotoxin a (LTa)–deficient embryos shows that LTa-mediated signaling is required to sustain proliferation and survival of stromal cells in vivo. Our data identify LTa-independent and LTa-dependent stages of lymph node development, and provide direct evidence for the role of LTic's during LN organogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. H. Ruddle and E. M. Akirav Secondary Lymphoid Organs: Responding to Genetic and Environmental Cues in Ontogeny and the Immune Response J. Immunol., August 15, 2009; 183(4): 2205 - 2212. [Abstract] [Full Text] [PDF] M. F. Vondenhoff, M. Greuter, G. Goverse, D. Elewaut, P. Dewint, C. F. Ware, K. Hoorweg, G. Kraal, and R. E. Mebius LT{beta}R Signaling Induces Cytokine Expression and Up-Regulates Lymphangiogenic Factors in Lymph Node Anlagen J. Immunol., May 1, 2009; 182(9): 5439 - 5445. [Abstract] [Full Text] [PDF] M. F. Vondenhoff, S. A. van de Pavert, M. E. Dillard, M. Greuter, G. Goverse, G. Oliver, and R. E. Mebius Lymph sacs are not required for the initiation of lymph node formation Development, January 1, 2009; 136(1): 29 - 34. [Abstract] [Full Text] [PDF]

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