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Blood, 15 October 2007, Vol. 110, No. 8, pp. 2872-2879. Prepublished online as a Blood First Edition Paper on June 21, 2007; DOI 10.1182/blood-2006-10-050583. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-10-050583v1 110/8/2872    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Li, G. Articles by Broxmeyer, H. E. Search for Related Content PubMed PubMed Citation Articles by Li, G. Articles by Broxmeyer, H. E. Related Collections Hematopoiesis and Stem Cells Immunobiology Chemokines, Cytokines, and Interleukins Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS TGF-ß combined with M-CSF and IL-4 induces generation of immune inhibitory cord blood dendritic cells capable of enhancing cytokine-induced ex vivo expansion of myeloid progenitors Geling Li1, Saeid Abediankenari1, Young-June Kim1, Timothy B. Campbell1, Shigeki Ito1, Barbara Graham-Evans1, Scott Cooper1, and Hal E. Broxmeyer1,2 1 Department of Microbiology and Immunology, Walther Oncology Center, Indiana University School of Medicine, and 2 Walther Cancer Institute, Indianapolis, IN Tolerogenic dendritic cells (DCs) may be valuable in transplantation for silencing immune reaction. Macrophage colony-stimulating factor (M-CSF)/IL-4 induces differentiation of cord blood (CB) monocytes into DCs (M-DCs) with tolerogenic phenotype/function. We assessed whether factors produced by tolerogenic DCs could modulate hematopoiesis. TGF-ß1 added to CB M-DC cultures induced bona fide DC morphology (TGF-M-DCs), similar to that of DCs generated with TGF-ß and granulocyte-macrophage colony-stimulating factor (GM-CSF)/IL-4 (TGF-GM-DCs). Of conditioned media (CM) produced from TGF-M-DCs, TGF-GM-DCs, M-DCs, and GM-DCs, TGF-M-DC CM was the only one that enhanced SCF, Flt3 ligand, and TPO expansion of myeloid progenitor cells ex vivo. This effect was blocked by neutralizing anti–M-CSF Ab, but protein analysis of CM suggested that M-CSF alone was not manifesting enhanced expansion of myeloid progenitors. LPS-stimulated TGF-M-DCs induced T-cell tolerance/anergy as effectively as M-DCs. TGF-M-DCs secreted significantly lower concentrations of progenitor cell inhibitory cytokines and were less potent in activating T cells than TGF-GM-DCs. Functional differences between TGF-M-DCs and TGF-GM-DCs included enhanced responses to LPS-induced ERK, JNK, and P38 activation in TGF-M-DCs and their immune suppressive–skewed cytokine release profiles. TGF-M-DCs appear unique among culture-generated DCs in their capability for silencing immunity while promoting expansion of myeloid progenitors, events that may be of therapeutic value. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Dendritic cells reach out in different directions David M. Bodine Blood 2007 110: 2783. [Full Text] [PDF] This article has been cited by other articles: R. D. Stout, S. K. Watkins, and J. Suttles Functional plasticity of macrophages: in situ reprogramming of tumor-associated macrophages J. Leukoc. Biol., November 1, 2009; 86(5): 1105 - 1109. [Abstract] [Full Text] [PDF]

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