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Blood, 15 June 2008, Vol. 111, No. 12, pp. 5467-5476. Prepublished online as a Blood First Edition Paper on April 18, 2008; DOI 10.1182/blood-2007-10-118547. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-10-118547v1 111/12/5467    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Burke, S. M. Articles by Marshall, J. S. Search for Related Content PubMed PubMed Citation Articles by Burke, S. M. Articles by Marshall, J. S. Related Collections Immunobiology Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism Sarah M. Burke1,2, Thomas B. Issekutz14, Karkada Mohan13, Patrick W. K. Lee2, Maya Shmulevitz2, and Jean S. Marshall1,2,4 1 Dalhousie Inflammation group, Departments of2 Microbiology and Immunology, 3 Pediatrics and 4 Pathology, Dalhousie University, Halifax, NS Human mast cells are found in skin and mucosal surfaces and next to blood vessels. They play a sentinel cell role in immunity, recognizing invading pathogens and producing proinflammatory mediators. Mast cells can recruit granulocytes, and monocytes in allergic disease and bacterial infection, but their ability to recruit antiviral effector cells such as natural killer (NK) cells and T cells has not been fully elucidated. To investigate the role of human mast cells in response to virus-associated stimuli, human cord blood–derived mast cells (CBMCs) were stimulated with polyinosinic·polycytidylic acid, a double-stranded RNA analog, or infected with the double-stranded RNA virus, reovirus serotype 3 Dearing for 24 hours. CBMCs responded to stimulation with polyinosinic·polycytidylic acid by producing a distinct chemokine profile, including CCL4, CXCL8, and CXCL10. CBMCs produced significant amounts of CXCL8 in response to low levels of reovirus infection, while both skin- and lung-derived fibroblasts were unresponsive unless higher doses of reovirus were used. Supernatants from CBMCs infected with reovirus induced substantial NK cell chemotaxis that was highly dependent on CXCL8 and CXCR1. These results suggest a novel role for mast cells in the recruitment of human NK cells to sites of early viral infection via CXCL8. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. G. Brown, Y. Y. Huang, J. S. Marshall, C. A. King, D. W. Hoskin, and R. Anderson Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection of human mast cells J. Leukoc. Biol., January 1, 2009; 85(1): 71 - 80. [Abstract] [Full Text] [PDF]

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