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Blood, 15 January 2008, Vol. 111, No. 2, pp. 699-704. Prepublished online as a Blood First Edition Paper on October 1, 2007; DOI 10.1182/blood-2007-05-088435. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-05-088435v1 111/2/699    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Biancotto, A. Articles by Grivel, J.-C. Search for Related Content PubMed PubMed Citation Articles by Biancotto, A. Articles by Grivel, J.-C. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY HIV-1–induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo Angélique Biancotto1, Sarah J. Iglehart1, Christophe Vanpouille1, Cristian E. Condack1, Andrea Lisco1, Elke Ruecker1, Ivan Hirsch2, Leonid B. Margolis1, and Jean-Charles Grivel1 1 Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, Bethesda, MD; and 2 Inserm UMR599, Centre de Recherche en Cancérologie de Marseille, Institut Paoli-Calmettes, Université Mediterranée, Marseille, France We demonstrate mechanisms by which HIV-1 appears to facilitate its own infection in ex vivo–infected human lymphoid tissue. In this system, HIV-1 readily infects various CD4+ T cells, but productive viral infection was supported predominantly by activated T cells expressing either CD25 or HLA-DR or both (CD25/HLA-DR) but not other activation markers: There was a strong positive correlation (r = 0.64, P = .001) between virus production and the number of CD25+/HLA-DR+ T cells. HIV-1 infection of lymphoid tissue was associated with activation of both HIV-1–infected and uninfected (bystanders) T cells. In these tissues, apoptosis was selectively increased in T cells expressing CD25/HLA-DR and p24gag but not in cells expressing either of these markers alone. In the course of HIV-1 infection, there was a significant increase in the number of activated (CD25+/HLA-DR+) T cells both infected and uninfected (bystander). By inducing T cells to express particular markers of activation that create new targets for infection, HIV-1 generates in ex vivo lymphoid tissues a vicious destructive circle of activation and infection. In vivo, such self-perpetuating cycle could contribute to HIV-1 disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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