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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1147-1156. Prepublished online as a Blood First Edition Paper on October 12, 2007; DOI 10.1182/blood-2007-07-099499. This Article Full Text Full Text (PDF) Supplemental Table and Figures Supplemental Videos Supplemental Videos All Versions of this Article: blood-2007-07-099499v1 111/3/1147    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kissa, K. Articles by Herbomel, P. Search for Related Content PubMed PubMed Citation Articles by Kissa, K. Articles by Herbomel, P. Related Collections Hematopoiesis and Stem Cells Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Live imaging of emerging hematopoietic stem cells and early thymus colonization Karima Kissa1, Emi Murayama1, Agustin Zapata2, Alfonso Cortés2, Emmanuelle Perret3, Christophe Machu3, and Philippe Herbomel1 1 Unité Macrophages et Développement de l'Immunité, Centre National de la Recherche Scientifique-Unité de Recherche Associée 2578, Institut Pasteur, Paris, France; 2 Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain; and 3 Plate forme d'Imagerie Dynamique, Institut Pasteur, Paris, France We recently demonstrated in zebrafish the developmental migration of emerging hematopoietic stem cells (HSCs) that is thought to occur in mammalian embryos, from the aorta-gonad-mesonephros (AGM) area to the successive hematopoietic organs. CD41 is the earliest known molecular marker of nascent HSCs in mammalian development. In this study, we show that in CD41-green fluorescent protein (GFP) transgenic zebrafish embryos, the transgene is expressed by emerging HSCs in the AGM, allowing us for the first time to image their behavior and trace them in real time. We find that the zebrafish AGM contains no intra-aortic cell clusters, so far considered a hallmark of HSC emergence. CD41GFPlow HSCs emerge in the subaortic mesenchyme and enter the circulation not through the dorsal aorta but through the axial vein, the peculiar structure of which facilitates their intravasation. The rise in CD41-gfp expression among c-myb+ HSC precursors is asynchronous and marks their competence to leave the AGM and immediately seed the caudal hematopoietic tissue (which has a hematopoietic function analogous to that of the mammalian fetal liver). Imaging the later migration of CD41-GFP+ precursors to the nascent thymus reveals that although some reach the thymus by extravasating from the nearest vein, most travel for hours through the mesenchyme from surprisingly diverse and remote sites of extravasation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Stem-cell trafficking at vascular borders Sarah Hutchinson and Nikolaus S. Trede Blood 2008 111: 975-976. [Full Text] [PDF] This article has been cited by other articles: M.-J. Yoon, B.-K. Koo, R. Song, H.-W. Jeong, J. Shin, Y.-W. Kim, Y.-Y. Kong, and P.-G. Suh Mind bomb-1 Is Essential for Intraembryonic Hematopoiesis in the Aortic Endothelium and the Subaortic Patches Mol. Cell. Biol., August 1, 2008; 28(15): 4794 - 4804. [Abstract] [Full Text] [PDF] J. Y. Bertrand, A. D. Kim, S. Teng, and D. Traver CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis Development, May 15, 2008; 135(10): 1853 - 1862. [Abstract] [Full Text] [PDF]

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