SEARCH:   Advanced

Blood, 1 February 2008, Vol. 111, No. 3, pp. 1157-1166. Prepublished online as a Blood First Edition Paper on October 17, 2007; DOI 10.1182/blood-2007-03-081323. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-03-081323v1 111/3/1157    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Walmsley, M. Articles by Patient, R. Search for Related Content PubMed PubMed Citation Articles by Walmsley, M. Articles by Patient, R. Related Collections Hematopoiesis and Stem Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Fibroblast growth factor controls the timing of Scl, Lmo2, and Runx1 expression during embryonic blood development Maggie Walmsley1, David Cleaver1, and Roger Patient1 1 Medical Research Council (MRC) Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom To program pluripotent cells into blood, a knowledge of the locations of precursors during their journey through the embryo and the signals they experience would be informative. The anterior (a) and posterior (p) ventral blood islands (VBIs) in Xenopus are derived from opposite sides of the pregastrula embryo. The aVBI goes through a "hemangioblast" state, characterized by coexpression of blood and endothelial genes at neurula stages, whereas the pVBI expresses these genes in a nonoverlapping fashion several hours later, after commitment to either a blood or an endothelial fate. We describe a novel role for fibroblast growth factor (FGF) in controlling the timing of Scl, Lmo2, and Runx1 expression in the 2 VBI compartments. Blocking FGF signaling during gastrulation expands expression at neurula stages into posterior regions. We show, by lineage labeling, explant analysis, and targeted blocking of FGF signaling, that this is due to the pVBI prematurely expressing these genes with the timing of the aVBI. In contrast, overexpression of FGF in aVBI precursors eliminates the anterior hemangioblast program. Using this information, we have recapitulated the anterior hemangioblast program in pluripotent cells in vitro by inhibiting FGF signaling in anterior mesoderm induced by activin and exposed to bone morphogenetic protein (BMP) signaling. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Chen, R. M. B. Costa, N. R. Love, X. Soto, M. Roth, R. Paredes, and E. Amaya C/EBP{alpha} initiates primitive myelopoiesis in pluripotent embryonic cells Blood, July 2, 2009; 114(1): 40 - 48. [Abstract] [Full Text] [PDF] T. Peterkin, A. Gibson, and R. Patient Common genetic control of haemangioblast and cardiac development in zebrafish Development, May 1, 2009; 136(9): 1465 - 1474. [Abstract] [Full Text] [PDF] R. M. B. Costa, X. Soto, Y. Chen, A. M. Zorn, and E. Amaya spib is required for primitive myeloid development in Xenopus Blood, September 15, 2008; 112(6): 2287 - 2296. [Abstract] [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020