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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1489-1496. Prepublished online as a Blood First Edition Paper on November 21, 2007; DOI 10.1182/blood-2007-07-102111. This Article Full Text Full Text (PDF) Supplemental Video All Versions of this Article: blood-2007-07-102111v1 111/3/1489    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kambayashi, T. Articles by Koretzky, G. A. Search for Related Content PubMed PubMed Citation Articles by Kambayashi, T. Articles by Koretzky, G. A. Related Collections Immunobiology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Indirect involvement of allergen-captured mast cells in antigen presentation Taku Kambayashi1,2, Jan D. Baranski3,4, Rebecca G. Baker1, Tao Zou1, Eric J. Allenspach5, Jonathan E. Shoag1, Peter L. Jones2,4, and Gary A. Koretzky1,2,5 1 Abramson Family Cancer Research Institute and 2 Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia; 3 Department of Bioengineering and 4 Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia; and 5 Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia It is generally thought that mast cells influence T-cell activation nonspecifically through the release of inflammatory mediators. In this report, we provide evidence that mast cells may also affect antigen-specific T-cell responses by internalizing immunoglobulin E–bound antigens for presentation to antigen-specific T cells. Surprisingly, T-cell activation did not require that mast cells express major histocompatibility complex class II, indicating that mast cells were not involved in the direct presentation of the internalized antigens. Rather, the antigen captured by mast cells is presented by other major histocompatibility complex class II+ antigen-presenting cells. To explore how this may occur, we investigated the fate of mast cells stimulated by antigen and found that FcRI crosslinking enhances mast cell apoptosis. Cell death by antigen-captured mast cells was required for efficient presentation because protection of mast cell death significantly decreased T-cell activation. These results suggest that mast cells may be involved in antigen presentation by acting as an antigen reservoir after antigen capture through specific immunoglobulin E molecules bound to their FcRI. This mechanism may contribute to how mast cells impact the development of T-cell responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Mast cells and T-cell expansion Susumu Nakae, Keisuke Oboki, and Hirohisa Saito Blood 2008 111: 2497-2498. [Full Text] [PDF] This article has been cited by other articles: T. Kambayashi, E. J. Allenspach, J. T. Chang, T. Zou, J. E. Shoag, S. L. Reiner, A. J. Caton, and G. A. Koretzky Inducible MHC Class II Expression by Mast Cells Supports Effector and Regulatory T Cell Activation J. Immunol., April 15, 2009; 182(8): 4686 - 4695. [Abstract] [Full Text] [PDF]

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