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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1617-1624. Prepublished online as a Blood First Edition Paper on November 16, 2007; DOI 10.1182/blood-2007-02-068791. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-02-068791v1 111/3/1617    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ortega-Paino, E. Articles by Borrebaeck, C. A. K. Search for Related Content PubMed PubMed Citation Articles by Ortega-Paino, E. Articles by Borrebaeck, C. A. K. Related Collections Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Functionally associated targets in mantle cell lymphoma as defined by DNA microarrays and RNA interference Eva Ortega-Paino1, Johan Fransson1, Sara Ek1, and Carl A. K. Borrebaeck1 1 Department of Immunotechnology, Lund University, Lund, Sweden Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma with poor prognosis. Its hallmark is the translocation t(11:14)q (13;32), leading to overexpression of cyclin D1, a positive regulator of the cell cycle. As cyclin D1 up-regulation is not sufficient for inducing malignant transformation, we combined DNA microarray and RNA interference (RNAi) approaches to identify novel deregulated genes involved in the progression of MCL. DNA microarray analysis identified 46 genes specifically up-regulated in MCL compared with normal B cells; 20 of these were chosen for further studies based on their cellular functions, such as growth and proliferation. The Granta 519 cell line was selected as an MCL in vitro model, to set up the RNAi protocol. To confirm the functionality of overexpression of the 20 disease-associated genes, they were knocked down using small interfering RNAs (siRNAs). In particular, knockdown of 3 genes, encoding the hepatoma-derived growth factor related protein 3 (HDGFRP3), the frizzled homolog 2 (FZD2), and the dual specificity phosphatase 5 (DUSP5), induced proliferative arrest in Granta 519 MCL cells. These genes emerged as functionally associated in MCL, in relation to growth and survival, and interfering with their function would increase insight into lymphoma growth regulation, potentially leading to novel clinical intervention modalities. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. M. El-Tahir, M. M. Abouzied, R. Gallitzendoerfer, V. Gieselmann, and S. Franken Hepatoma-derived Growth Factor-related Protein-3 Interacts with Microtubules and Promotes Neurite Outgrowth in Mouse Cortical Neurons J. Biol. Chem., April 24, 2009; 284(17): 11637 - 11651. [Abstract] [Full Text] [PDF] T. Boutros, E. Chevet, and P. Metrakos Mitogen-Activated Protein (MAP) Kinase/MAP Kinase Phosphatase Regulation: Roles in Cell Growth, Death, and Cancer Pharmacol. Rev., September 1, 2008; 60(3): 261 - 310. [Abstract] [Full Text] [PDF]

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