SEARCH:   Advanced

Blood, 15 April 2008, Vol. 111, No. 8, pp. 3978-3985. Prepublished online as a Blood First Edition Paper on December 21, 2007; DOI 10.1182/blood-2007-10-119636. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-10-119636v1 111/8/3978    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Walker, F. Articles by Burgess, A. W. PubMed PubMed Citation Articles by Walker, F. Articles by Burgess, A. W. Related Collections Chemokines, Cytokines, and Interleukins Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF– and GM-CSF–deficient mice Francesca Walker1, Hui-Hua Zhang1, Vance Matthews2, Janet Weinstock1, Edouard C. Nice1, Matthias Ernst1, Stefan Rose-John3, and Antony W. Burgess1 1 Ludwig Institute for Cancer Research, Melbourne Tumor Biology Branch, Melbourne, Australia; 2 Baker Heart Research Institute, Melbourne, Australia; and 3 Biochemisches Institute, Christian-Albrechts-Universitat Universität zu Kiel, Kiel, Germany Mice defective in both granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) have severely impaired neutrophil production and function, yet these mice respond to acute pathogen challenge with a significant neutrophil response. We have recently reported the development of an in vitro system to detect granulopoietic cytokines secreted from cells isolated from G-CSF, GM-CSF double knockout mice. The conditioned media produced by these cells after stimulation with lipopolysaccharide or Candida albicans supports the production and differentiation of granulocytes (ie, the conditioned media contains neutrophil promoting activity [NPA]). We now show that the NPA in the G-CSF–/–/GM-CSF–/– conditioned media requires interleukin-6 (IL6), is abolished by soluble gp130, and can be specifically immunodepleted by an anti-IL6R antibody. NPA effects on bone marrow cells are also mimicked by Hyper-IL6, and the soluble IL6R is present in NPA. These results show that the IL6/sIL6R complex is the major effector of NPA. NPA production by mice defective for both G-CSF and GM-CSF uncovers an alternative pathway to granulocyte production, which is activated after exposure to pathogens. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: IL6 to the rescue Peter E. Newburger Blood 2008 111: 3914-3915. [Full Text] [PDF]

	Blood Online is supported in part by Genentech BioOncology and Biogen Idec
	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020