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Blood, 15 April 2008, Vol. 111, No. 8, pp. 4220-4232. Prepublished online as a Blood First Edition Paper on November 26, 2007; DOI 10.1182/blood-2007-07-101691. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-07-101691v1 111/8/4220    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Chen, Y. Articles by Chen, Z. W. PubMed PubMed Citation Articles by Chen, Y. Articles by Chen, Z. W. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY NSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal V2V2 T-cell expansion Yong Chen1, Lingyun Shao1,2, Zahida Ali1, Jiye Cai1,3, and Zheng W. Chen1 1 Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago; 2 Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China; and 3 Department of Chemistry, Jinan University, Guangzhou, Guangdong, China Nanoscale imaging of an in vivo antigen-specific T-cell immune response has not been reported. Here, the combined near-field scanning optical microscopy– and fluorescent quantum dot–based nanotechnology was used to perform immunofluorescence imaging of antigen-specific T-cell receptor (TCR) response in an in vivo model of clonal T-cell expansion. The near-field scanning optical microscopy/quantum dot system provided a best-optical-resolution (<50 nm) nano-scale imaging of V2V2 TCR on the membrane of nonstimulated V2V2 T cells. Before Ag-induced clonal expansion, these nonstimulating V2V2 TCRs appeared to be distributed differently from their β TCR counterparts on the cell surface. Surprisingly, V2V2 TCR nanoclusters not only were formed but also sustained on the membrane during an in vivo clonal expansion of V2V2 T cells after phosphoantigen treatment or phosphoantigen plus mycobacterial infection. The TCR nanoclusters could array to form nanodomains or microdomains on the membrane of clonally expanded V2V2 T cells. Interestingly, expanded V2V2 T cells bearing TCR nanoclusters or nanodomains were able to rerecognize phosphoantigen and to exert better effector function. These studies provided nanoscale insight into the in vivo T-cell immune response. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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