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Blood, 1 December 2008, Vol. 112, No. 12, pp. 4729-4738. Prepublished online as a Blood First Edition Paper on September 16, 2008; DOI 10.1182/blood-2008-05-159269. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-05-159269v1 112/12/4729    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Montel-Hagen, A. Articles by Taylor, N. Search for Related Content PubMed PubMed Citation Articles by Montel-Hagen, A. Articles by Taylor, N. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis Amélie Montel-Hagen1, Lionel Blanc2, Myriam Boyer-Clavel1, Chantal Jacquet1, Michel Vidal2, Marc Sitbon1, and Naomi Taylor1 1 Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 5535/Institut Fédératif de Recherche 122 (IFR122); and 2 Dynamique des Interactions Membranaires et Pathologiques (DIMNP), CNRS UMR 5235, Université Montpellier I and II, Montpellier, France Glucose is a major source of energy for living organisms, and its transport in vertebrates is a universally conserved property. Of all cell lineages, human erythrocytes express the highest level of the Glut1 glucose transporter with more than 200 000 molecules per cell. However, we recently reported that erythrocyte Glut1 expression is a specific trait of vitamin C–deficient mammalian species, comprising only higher primates, guinea pigs, and fruit bats. Here, we show that in all other tested mammalian species, Glut1 was transiently expressed in erythrocytes during the neonatal period. Glut1 was up-regulated during the erythroblast stage of erythroid differentiation and was present on the vast majority of murine red blood cells (RBCs) at birth. Notably though, Glut1 was not induced in adult mice undergoing anemia-induced erythropoiesis, and under these conditions, the up-regulation of a distinct transporter, Glut4, was responsible for an increased glucose transport. Sp3 and Sp1 transcriptions factors have been proposed to regulate Glut1 transcription, and we find that the concomitant repression of Glut1 and induction of Glut4 was associated with a significantly augmented Sp3/Sp1 ratio. Glucose transporter expression patterns in mice and human erythrocytes are therefore distinct. In mice, there is a postnatal switch from Glut1 to Glut4, with Glut4 further up-regulated under anemic conditions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Blanc, J. Liu, M. Vidal, J. A. Chasis, X. An, and N. Mohandas The water channel aquaporin-1 partitions into exosomes during reticulocyte maturation: implication for the regulation of cell volume Blood, October 29, 2009; 114(18): 3928 - 3934. [Abstract] [Full Text] [PDF]

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