Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 15 August 2008, Vol. 112, No. 4, pp. 1184-1194.
Prepublished online as a Blood First Edition Paper on June 5, 2008; DOI 10.1182/blood-2007-12-127951.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Figures
Right arrow All Versions of this Article:
blood-2007-12-127951v1
112/4/1184    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ju, X.
Right arrow Articles by Clark, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ju, X.
Right arrow Articles by Clark, G. J.
Related Collections
Right arrow Immunobiology
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

IMMUNOBIOLOGY

CD300a/c regulate type I interferon and TNF-{alpha} secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands

Xinsheng Ju1, Martin Zenke2,3, Derek N. J. Hart1, and Georgina J. Clark1

1 Mater Medical Research Institute, Aubigny Place, Brisbane, Australia; 2 Institute for Biomedical Engineering, Department of Cell Biology, Aachen University Hospital, Aachen, Germany; and 3 Helmholtz Institute for Biomedical Engineering, Rheinisch-Westfälische Technische Hochschule, Aachen, Germany

Activation of human plasmacytoid dendritic cells (pDCs) with ligands for Toll-like receptors (TLRs) 7 and 9 induces the secretion of type I interferons and other inflammatory cytokines as well as pDC differentiation. Transcripts for 2 members of the CD300 gene family, CD300a and CD300c, were identified on pDCs during gene expression studies to identify new immunoregulatory molecules on pDCs. We therefore investigated the expression of CD300a and CD300c and their potential regulation of pDC function. CD300a/c RNA and surface expression were downregulated after stimulation of pDCs with TLR7 and TLR9 ligands. Exogenous interferon (IFN)-{alpha} down-regulated CD300a/c expression, whereas neutralizing IFN-{alpha} abolished TLR ligand–induced CD300a/c down-regulation. This implicates IFN-{alpha} in regulating CD300a/c expression in pDCs. In addition, IFN-{alpha} favored tumor necrosis factor (TNF)-{alpha} secretion by CpG-induced pDCs. CD300a/c triggering by cross-linking antibody reduced TNF-{alpha} and increased IFN-{alpha} secretion by pDCs. Furthermore, CD300a/c triggering, in the presence of neutralizing IFN-{alpha}, further reduced TNF-{alpha} secretion. These data indicate that CD300a and CD300c play an important role in the cross-regulation of TNF-{alpha} and IFN-{alpha} secretion from pDCs.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020