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Blood, 15 September 2008, Vol. 112, No. 6, pp. 2529-2538. Prepublished online as a Blood First Edition Paper on June 25, 2008; DOI 10.1182/blood-2008-01-132506. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-01-132506v1 112/6/2529    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nandedkar, S. D. Articles by Pritchard, K. A. Search for Related Content PubMed PubMed Citation Articles by Nandedkar, S. D. Articles by Pritchard, K. A. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS Histopathology of experimentally induced asthma in a murine model of sickle cell disease Sandhya D. Nandedkar1, Thomas R. Feroah2, William Hutchins2, Dorothee Weihrauch3, Kameswari S. Konduri1, Jingli Wang1, Robert C. Strunk4, Michael R. DeBaun4, Cheryl A. Hillery5,*, and Kirkwood A. Pritchard1,* 1 Department of Surgery, Division of Pediatric Surgery, 2 Department of Pediatrics, Division of Pulmonary/Critical Care, and 3 Department of Anesthesiology, Medical College of Wisconsin, Blood Research Institute, Children's Research Institute, Cardiovascular Center, Milwaukee, WI; 4 Department of Pediatrics, Division of Allergy and Pulmonary Medicine and Division of Genetics, Washington University School of Medicine, St Louis, MO; and 5 Department of Pediatrics, Division of Hematology/Oncology, Children's Research Institute, Cardiovascular Center, Milwaukee, WI Asthma is a comorbid condition associated with increased rates of pain, acute chest syndrome, and premature death in human sickle cell disease (SCD). We developed an experimental asthma model in SCD and control mice expressing either normal human or murine hemoglobin to determine its effect on mortality and lung pathology. To induce lung inflammation, experimental mice were sensitized to ovalbumin (OVA) by subcutaneous OVA implantation (Sen), allowed 2 weeks to recover, and then divided into 2 groups, each receiving over a subsequent 10-day period the same dosage of aerosolized OVA but 2 different levels of exposure: 15 minutes (LoSen) and 30 minutes (HiSen). During recovery, 10% of SCD mice died compared with no deaths in control mice. An additional 30% of HiSen SCD mice died during aerosolization compared with 10% in LoSen SCD. Histologic indices of lung inflammation (eg, eosinophil recruitment, airway and vessel wall thickening, and immunoreactive TGFβ and fsp-1) and bronchial alveolar lavage fluid eosinophil peroxidase activity differentially increased in sensitized mice compared with unsensitized mice. Our findings indicate SCD mice with experimentally induced asthma are more susceptible to death and pulmonary inflammation compared with control mice, suggesting that asthma contributes significantly to morbidity and mortality in SCD. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Patel, C. S. Gonsalves, M. Yang, P. Malik, and V. K. Kalra Placenta growth factor induces 5-lipoxygenase-activating protein to increase leukotriene formation in sickle cell disease Blood, January 29, 2009; 113(5): 1129 - 1138. [Abstract] [Full Text] [PDF]

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