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Blood, 1 November 2008, Vol. 112, No. 9, pp. 3540-3541.
Why is CLL refractory to bortezomib?ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL
In this issue of Blood, Liu and colleagues present evidence that flavonoids present in plasma may compromise the ability of the proteasome inhibitor bortezomib to induce apoptosis of CLL cells.
The blocking effect of flavonoids is attributable to a direct chemical reaction between the boronate moiety of bortezomib and adjacent hydroxyl groups present on the B ring of some, but not all, flavonoids. First, quercetin and myricetin, which contain adjacent hydroxyls, effectively blocked bortezomib's cytotoxic action, while kaempferol and apigenin, which do not contain adjacent hydroxyls, failed to do so. Second, cell killing by the proteasome inhibitors MG-132 and lactacystin, proteasome inhibitors which do not possess a boronate group, was unaffected by flavonoids, which compromised apoptosis induction by the boronate compounds, bortezomib and MG-262. Finally, data obtained using Raman spectroscopy were consistent with a direct chemical reaction between quercetin and bortezomib. Liu et al also show that boric acid reacts with quercetin and abolishes its inhibitory action on bortezomib-induced apoptosis. Boric acid also abrogated the protective action of plasma, suggesting that the ability of plasma to compromise CLL cell killing by bortezomib is indeed attributable to flavonoids. However, questions remain concerning the actual levels of flavonoid species present in plasma. While plasma levels of quercetin are insufficient on their own to account for the quenching effect, other flavonoid species are present in plasma. It would therefore be of interest to identify and quantify those species which are reactive with bortezomib. The concentration issue is particularly relevant since the actions of quercetin are significantly dose-dependent: While 20 µM quercetin effectively blocked killing of CLL cells by bortezomib, concentrations of 40 µM or greater actually induced apoptosis, an action that may be explained by the apparently paradoxical observation that quercetin itself may, like bortezomib, inhibit the β5 subunit of the proteasome.3 The studies here raise a further question: If plasma flavonoids effectively neutralize the cytotoxic actions of bortezomib on CLL cells, how is the observed effectiveness of this agent in multiple myeloma4 explained? The authors suggest that interactions between flavonoids and CLL cells on the one hand and myeloma cells on the other may be different. Specifically, they propose that quercetin may augment the sensitivity of multiple myeloma cells to bortezomib, so as to compensate for chemical inactivation of the proteasome inhibitor by the flavonoid. It would be of interest to explore this possibility via comparative mechanistic studies on CLL and myeloma cells. The authors suggest several strategies which may allow further development of proteasome inhibitors for the treatment of CLL. First, decreasing plasma flavonoid concentrations by dietary manipulation may, if achievable, be of value in enhancing in vivo toxicity of bortezomib. Second, the observation that boric acid can compete with the reaction between quercetin and bortezomib raises the possibility that the blocking effect of fla-vonoids may be neutralized prior to bor-tezomib treatment. A third option would be to explore the possible therapeutic use of proteasome inhibitors which lack a boronate moiety. In some respects, the data of Liu et al echo earlier observations that plasma blocked apoptosis induction of CLL cells following treatment with chlorambucil.5 However, the mechanism of blockade in this earlier study was different than that described here and involved inhibition of intracellular apoptotic-signaling pathways by albumin following induction of DNA damage, rather than by direct inactivation of chlorambucil by reaction with plasma components. Nevertheless, these stud-ies emphasize the sometimes profound effects of human plasma on cytotoxic mechanisms and open the way for the design of strategies which may result in more effective therapeutic use of existing cytotoxic agents.
Footnotes
Conflict-of-interest disclosure: The author declares no competing financial interests.
REFERENCES
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| Copyright © 2008 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
