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Blood, 1 January 2009, Vol. 113, No. 1, pp. 75-84. Prepublished online as a Blood First Edition Paper on September 25, 2008; DOI 10.1182/blood-2008-02-139865. This Article Full Text Full Text (PDF) Supplemental Table, Figures, and Videos All Versions of this Article: blood-2008-02-139865v1 113/1/75    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mittelbrunn, M. Articles by Sánchez-Madrid, F. Search for Related Content PubMed PubMed Citation Articles by Mittelbrunn, M. Articles by Sánchez-Madrid, F. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Imaging of plasmacytoid dendritic cell interactions with T cells María Mittelbrunn1,*, Gloria Martínez del Hoyo1,*, María López-Bravo2, Noa B. Martín-Cofreces3, Alix Scholer4, Stéphanie Hugues4, Luc Fetler5, Sebastián Amigorena4, Carlos Ardavín2, and Francisco Sánchez-Madrid1,3 1 Departamento de Biología Vascular e Inflamación, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain; 2 Departamento de Inmunología y Oncología, Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Madrid, Spain; 3 Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain; 4 INSERM Unite Mixte de Recherche 653, Immunité et Cancer, Pavillon Pasteur, Institut Curie, Paris, France; and 5 Centre National de la Recherche Scientifique, Unite Mixte de Recherche 168, Laboratoire de Physico-Chimie Curie, Institut Curie, Paris, France Plasmacytoid dendritic cells (pDCs) efficiently produce type I interferon and participate in adaptive immune responses, although the molecular interactions between pDCs and antigen-specific T cells remain unknown. This study examines immune synapse (IS) formation between murine pDCs and CD4+ T cells. Mature pDCs formed canonical ISs, involving relocation to the contact site of the microtubule-organizing center, F-actin, protein kinase C-, and pVav, and activation of early signaling molecules in T cells. However, immature pDCs were less efficient at forming conjugates with T cells and inducing IS formation, microtubule-organizing center translocation, and T-cell signaling and activation. Time-lapse videomicroscopy and 2-photon in vivo imaging of pDC–T-cell interactions revealed that immature pDCs preferentially mediated transient interactions, whereas mature pDCs promoted more stable contacts. Our data indicate that, under steady-state conditions, pDCs preferentially establish transient contacts with naive T cells and show a very modest immunogenic capability, whereas on maturation, pDCs are able to form long-lived contacts with T cells and significantly enhance their capacity to activate these lymphocytes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Teshima, M. Kadowaki, and M. Koyama Response: Recipient plasmacytoid dendritic cells and graft-versus-host disease Blood, August 6, 2009; 114(6): 1280 - 1280. [Full Text] [PDF] M. Koyama, D. Hashimoto, K. Aoyama, K.-i. Matsuoka, K. Karube, H. Niiro, M. Harada, M. Tanimoto, K. Akashi, and T. Teshima Plasmacytoid dendritic cells prime alloreactive T cells to mediate graft-versus-host disease as antigen-presenting cells Blood, February 26, 2009; 113(9): 2088 - 2095. [Abstract] [Full Text] [PDF]

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