Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 16 April 2009, Vol. 113, No. 16, pp. 3716-3725.
Prepublished online as a Blood First Edition Paper on November 18, 2008; DOI 10.1182/blood-2008-09-179754.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Table and Figures
Right arrow All Versions of this Article:
blood-2008-09-179754v1
113/16/3716    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bruhns, P.
Right arrow Articles by Daëron, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bruhns, P.
Right arrow Articles by Daëron, M.
Related Collections
Right arrow Immunobiology
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

IMMUNOBIOLOGY

Specificity and affinity of human Fc{gamma} receptors and their polymorphic variants for human IgG subclasses

Pierre Bruhns1,2, Bruno Iannascoli1,2, Patrick England3,4, David A. Mancardi1,2, Nadine Fernandez5, Sylvie Jorieux6, and Marc Daëron1,2

1 Institut Pasteur, Département d'Immunologie, Unité d'Allergologie Moléculaire et Cellulaire, Paris; 2 Inserm, U760, Paris; 3 Institut Pasteur, Département de Biologie Structurale et Chimie, Plateforme de Biophysique des Macromolécules et de leurs Interactions, Paris; 4 Centre National de la Recherche Scientifique (CNRS), URA 2185, Paris; 5 Laboratoire Français du fractionnement et des Biotechnologies, Les Ulis; and 6 Laboratoire Français du fractionnement et des Biotechnologies, Lille, France

Distinct genes encode 6 human receptors for IgG (hFc{gamma}Rs), 3 of which have 2 or 3 polymorphic variants. The specificity and affinity of individual hFc{gamma}Rs for the 4 human IgG subclasses is unknown. This information is critical for antibody-based immunotherapy which has been increasingly used in the clinics. We investigated the binding of polyclonal and monoclonal IgG1, IgG2, IgG3, and IgG4 to Fc{gamma}RI; Fc{gamma}RIIA, IIB, and IIC; Fc{gamma}RIIIA and IIIB; and all known polymorphic variants. Wild-type and low-fucosylated IgG1 anti-CD20 and anti-RhD mAbs were also examined. We found that (1) IgG1 and IgG3 bind to all hFc{gamma}Rs; (2) IgG2 bind not only to Fc{gamma}RIIAH131, but also, with a lower affinity, to Fc{gamma}RIIAR131 and Fc{gamma}RIIIAV158; (3) IgG4 bind to Fc{gamma}RI, Fc{gamma}RIIA, IIB and IIC and Fc{gamma}RIIIAV158; and (4) the inhibitory receptor Fc{gamma}RIIB has a lower affinity for IgG1, IgG2, and IgG3 than all other hFc{gamma}Rs. We also identified parameters that determine the specificity and affinity of hFc{gamma}Rs for IgG subclasses. These results document how hFc{gamma}R specificity and affinity may account for the biological activities of antibodies. They therefore highlight the role of specific hFc{gamma}Rs in the therapeutic and pathogenic effects of antibodies in disease.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
M. Lazarou, J. A. G. Patino, R. M. Jennings, R. S. McIntosh, J. Shi, S. Howell, E. Cullen, T. Jones, J. R. Adame-Gallegos, J. A. Chappel, et al.
Inhibition of Erythrocyte Invasion and Plasmodium falciparum Merozoite Surface Protein 1 Processing by Human Immunoglobulin G1 (IgG1) and IgG3 Antibodies
Infect. Immun., December 1, 2009; 77(12): 5659 - 5667.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Hudrisier, B. Clemenceau, S. Balor, S. Daubeuf, E. Magdeleine, M. Daeron, P. Bruhns, and H. Vie
Ligand Binding but Undetected Functional Response of FcR after Their Capture by T Cells via Trogocytosis
J. Immunol., November 15, 2009; 183(10): 6102 - 6113.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. G. Perez, M. R. Costa, C. A. Todd, B. F. Haynes, and D. C. Montefiori
Utilization of Immunoglobulin G Fc Receptors by Human Immunodeficiency Virus Type 1: a Specific Role for Antibodies against the Membrane-Proximal External Region of gp41
J. Virol., August 1, 2009; 83(15): 7397 - 7410.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020