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Blood, 23 April 2009, Vol. 113, No. 17, pp. 3999-4007. Prepublished online as a Blood First Edition Paper on December 4, 2008; DOI 10.1182/blood-2008-03-145979. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-03-145979v1 113/17/3999    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jackson, S. M. Articles by Capra, J. D. Search for Related Content PubMed PubMed Citation Articles by Jackson, S. M. Articles by Capra, J. D. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Key developmental transitions in human germinal center B cells are revealed by differential CD45RB expression Stephen M. Jackson1,2, Natessa Harp1, Darshna Patel1, Jordan Wulf1, Erich D. Spaeth1, Uzoamaka K. Dike1, Judith A. James2,3, and J. Donald Capra1 1 Molecular Immunogenetics Research Program and 2 Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City; and 3 Department of Medicine and Pathology, University of Oklahoma Health Science Center, Oklahoma City We previously reported that RO+ expression correlated with increased mutation, activation, and selection among human germinal center (GC) B cells. Here, we subdivided human tonsillar B cells, including IgD–CD38+ GC B cells, into different fractions based on RB expression. Although each subset contained RB+ cells, when used as an intrasubset marker, differential RB expression effectively discriminated between phenotypically distinct cells. For example, RB+ GC B cells were enriched for activated cells with lower AID expression. RB inversely correlated with mutation frequency, demonstrating a key difference between RB- and RO-expressing GC B cells. Reduced RB expression during the transition from pre-GC (IgM+IgD+CD38+CD27–) to GCB cells was followed by a dramatic increase during the GC-to-plasmablast (IgD–CD38++CD27+) and memory (IgD–CD38–CD27+) transition. Interestingly, RB+ GC B cells showed increased signs of terminal differentiation toward CD27+ post-GC early plasmablast (increased CD38 and RO) or early memory (decreased CD38 and RO) B cells. We propose that as in T cells, differential RB expression directly correlates with development- and function-based transitions in tonsillar B cells. Application of this RB:RO system should advance our understanding of normal B-cell development and facilitate the isolation of more discrete B-cell populations with potentially different propensities in disease pathogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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