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Blood, 28 May 2009, Vol. 113, No. 22, pp. 5488-5496. Prepublished online as a Blood First Edition Paper on February 20, 2009; DOI 10.1182/blood-2008-10-187179. This Article Full Text Full Text (PDF) Supplemental Tables and Figure All Versions of this Article: blood-2008-10-187179v1 113/22/5488    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chiossone, L. Articles by Walzer, T. Search for Related Content PubMed PubMed Citation Articles by Chiossone, L. Articles by Walzer, T. Related Collections Hematopoiesis and Stem Cells Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Maturation of mouse NK cells is a 4-stage developmental program Laura Chiossone13, Julie Chaix13, Nicolas Fuseri4, Claude Roth5, Eric Vivier13,6, and Thierry Walzer13 1 Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille; 2 Inserm, U631, Marseille; 3 Centre National de la Recherche Scientifique (CNRS), UMR6102, Marseille; 4 Innate-Pharma, Marseille; 5 Département d'Immunologie, Institut Pasteur, Paris; and 6 Assistance Publique-Hôpitaux (AP-HP) de Marseille, Hôpital de la Conception, Marseille, France Surface density of CD27 and CD11b subdivides mouse natural killer (NK) cells into 4 subsets: CD11blowCD27low, CD11blowCD27high, CD11bhighCD27high, and CD11bhighCD27low. To determine the developmental relationship between these 4 subsets, we used several complementary approaches. First, we took advantage of NDE transgenic mice that express enhanced green fluorescent protein (EGFP) and diphtheria toxin receptor specifically in NK cells. Diphtheria toxin injection leads to a transient depletion of NK cells, allowing the monitoring of the phenotype of developing EGFP+ NK cells after diphtheria toxin injection. Second, we evaluated the overall proximity between NK-cell subsets based on their global gene profile. Third, we compared the proliferative capacity of NK-cell subsets at steady state or during replenishment of the NK-cell pool. Fourth, we performed adoptive transfers of EGFP+ NK cell subsets from NDE mice into unirradiated mice and followed the fate of transferred cells. The results of these various experiments collectively support a 4-stage model of NK-cell maturation CD11blowCD27low CD11blowCD27high CD11bhighCD27high CD11bhighCD27low. This developmental program appears to be associated with a progressive acquisition of NK-cell effector functions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. G. Hoover, G. Gullickson, and J. Kornbluth Impaired NK Cytolytic Activity and Enhanced Tumor Growth in NK Lytic-Associated Molecule-Deficient Mice J. Immunol., December 1, 2009; 183(11): 6913 - 6921. [Abstract] [Full Text] [PDF] T. Zhang, S. Liu, P. Yang, C. Han, J. Wang, J. Liu, Y. Han, Y. Yu, and X. Cao Fibronectin maintains survival of mouse natural killer (NK) cells via CD11b/Src/{beta}-catenin pathway Blood, November 5, 2009; 114(19): 4081 - 4088. [Abstract] [Full Text] [PDF]

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