Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 11 June 2009, Vol. 113, No. 24, pp. 6138-6147.
Prepublished online as a Blood First Edition Paper on January 12, 2009; DOI 10.1182/blood-2008-07-167668.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Figures and Videos
Right arrow All Versions of this Article:
blood-2008-07-167668v1
113/24/6138    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Gérard, A.
Right arrow Articles by Collard, J. G.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gérard, A.
Right arrow Articles by Collard, J. G.
Related Collections
Right arrow Immunobiology
Right arrow Vascular Biology
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

IMMUNOBIOLOGY

The Rac activator Tiam1 controls efficient T-cell trafficking and route of transendothelial migration

Audrey Gérard1, Rob A. van der Kammen1, Hans Janssen2, Saskia I. Ellenbroek1, and John G. Collard1

Divisions of 1 Cell Biology and 2 Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands

Migration toward chemoattractants is a hallmark of T-cell trafficking and is essential to produce an efficient immune response. Here, we have analyzed the function of the Rac activator Tiam1 in the control of T-cell trafficking and transendothelial migration. We found that Tiam1 is required for chemokine- and S1P-induced Rac activation and subsequent cell migration. As a result, Tiam1-deficient T cells show reduced chemotaxis in vitro, and impaired homing, egress, and contact hypersensitivity in vivo. Analysis of the T-cell transendothelial migration cascade revealed that PKC{zeta}/Tiam1/Rac signaling is dispensable for T-cell arrest but is essential for the stabilization of polarization and efficient crawling of T cells on endothelial cells. T cells that lack Tiam1 predominantly transmigrate through individual endothelial cells (transcellular migration) rather than at endothelial junctions (paracellular migration), suggesting that T cells are able to change their route of transendothelial migration according to their polarization status and crawling capacity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 JEMHome page
Z. Mamdouh, A. Mikhailov, and W. A. Muller
Transcellular migration of leukocytes is mediated by the endothelial lateral border recycling compartment
J. Exp. Med., November 23, 2009; 206(12): 2795 - 2808.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. V. Carman
Mechanisms for transcellular diapedesis: probing and pathfinding by `invadosome-like protrusions'
J. Cell Sci., September 1, 2009; 122(17): 3025 - 3035.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020