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Blood, 5 February 2009, Vol. 113, No. 6, pp. 1257-1267. Prepublished online as a Blood First Edition Paper on November 5, 2008; DOI 10.1182/blood-2008-06-165266. This Article Full Text Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2008-06-165266v1 113/6/1257    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Angel, C. E. Articles by Dunbar, P. R. Search for Related Content PubMed PubMed Citation Articles by Angel, C. E. Articles by Dunbar, P. R. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Distinctive localization of antigen-presenting cells in human lymph nodes Catherine E. Angel1, Chun-Jen J. Chen1, Oliver C. Horlacher1, Sintia Winkler1, Thomas John2, Judy Browning2, Duncan MacGregor2, Jonathan Cebon2, and P. Rod Dunbar1,3 1 School of Biological Sciences, University of Auckland, Auckland, New Zealand; 2 Ludwig Institute for Cancer Research, Austin Health, Heidelberg, Victoria, Australia; and 3 Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand Professional antigen-presenting cells (APCs) are sentinel cells of the immune system that present antigen to T lymphocytes and mediate an appropriate immune response. It is therefore surprising that knowledge of the professional APCs in human lymph nodes is limited. Using 3-color immunohistochemistry, we have identified APCs in human lymph nodes, excluding plasmacytoid APCs, that fall into 2 nonoverlapping classes: (1) CD209+ APCs, coexpressing combinations of CD206, CD14, and CD68, that occupied the medullary cords, lined the capsule and trabeculae and were also scattered throughout the diffuse T-lymphocyte areas of the paracortex; and (2) APCs expressing combinations of CD1a, CD207, and CD208, that were always restricted to the paracortex. Surprisingly, this second class of APCs was almost entirely absent from many lymph nodes. Our data suggest that most CD208+ cells, often referred to as "interdigitating cells," derive from migratory APCs, and that the major APC subset consistently resident in the paracortex of human lymph nodes is the CD209+ subset. All APC subsets were demonstrated to be in close contact with the fibroreticular network. The identification of 2 distinct APC populations in the paracortex of human lymph nodes has important implications for understanding T-lymphocyte responses and optimizing vaccine design. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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