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Blood, 2 July 2009, Vol. 114, No. 1, pp. 109-118. Prepublished online as a Blood First Edition Paper on May 5, 2009; DOI 10.1182/blood-2008-12-193375. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-12-193375v1 114/1/109    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Tsai, S.-C. Articles by Tsai, C.-H. PubMed PubMed Citation Articles by Tsai, S.-C. Articles by Tsai, C.-H. Related Collections Lymphoid Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  LYMPHOID NEOPLASIA EBV Zta protein induces the expression of interleukin-13, promoting the proliferation of EBV-infected B cells and lymphoblastoid cell lines Shu-Chun Tsai1, Sue-Jane Lin1,2, Po-Wen Chen1, Wen-Yi Luo1, Te-Huei Yeh3, Hsei-Wei Wang4, Chi-Ju Chen4, and Ching-Hwa Tsai1 1 Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei; 2 Immunology Research Center, National Health Research Institutes, Miaoli; 3 Department of Otolaryngology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei; and 4 Department and Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan Epstein-Barr virus (EBV) infection can modify the cytokine expression profiles of host cells and determine the fate of those cells. Of note, expression of interleukin-13 (IL-13) may be detected in EBV-associated Hodgkin lymphoma and the natural killer (NK) cells of chronic active EBV-infected patients, but its biologic role and regulatory mechanisms are not understood. Using cytokine antibody arrays, we found that IL-13 production is induced in B cells early during EBV infection. Furthermore, the EBV lytic protein, Zta (also known as the BZLF-1 product), which is a transcriptional activator, was found to induce IL-13 expression following transfection. Mechanistically, induction of IL-13 expression by Zta is mediated directly through its binding to the IL-13 promoter, via a consensus AP-1 binding site. Blockade of IL-13 by antibody neutralization showed that IL-13 is required at an early stage of EBV-induced proliferation and for long-term maintenance of the growth of EBV immortalized lymphoblastoid cell lines (LCLs). Thus, Zta-induced IL-13 production facilitates B-cell proliferation and may contribute to the pathogenesis of EBV-associated lymphoproliferative disorders, such as posttransplantation lymphoproliferative disease (PTLD) and Hodgkin lymphoma. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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