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Prepublished online as a Blood First Edition Paper on May 13, 2002; DOI 10.1182/blood-2001-11-0005. This Article Full Text (PDF) All Versions of this Article: 2001-11-0005v1 100/5/1915    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Eyrich, M. Articles by Schlegel, P. G Search for Related Content PubMed PubMed Citation Articles by Eyrich, M. Articles by Schlegel, P. G Social Bookmarking                   What's this? Submitted December 18, 2001 Accepted April 15, 2002 Distinct contributions of CD4+ and CD8+ naive and memory T-cell subsets to overall TCR-repertoire complexity following transplantation of T-cell depleted CD34 selected hematopoietic progenitor cells from unrelated donors Matthias Eyrich, Tanja Croner, Christine Leiler, Peter Lang, Peter Bader, Thomas Klingebiel, Dietrich Niethammer, and Paul G Schlegel* Pediatric Stem Cell Transplant Program, University of Tuebingen, Tuebingen, Germany Department of Pediatric Hematology/Oncology, University of Frankfurt, University Children's Hospital, Frankfurt, Germany * Corresponding author; email: p-g.schlegel{at}med.uni-tuebingen.de. Normalization of restricted T-cell receptor (TCR) repertoire is critical following T-cell-depleted (TCD) stem cell transplantation. We present a prospective study analyzing respective contributions of naive and memory T-cell subsets within the CD4+ and CD8+ compartments to the evolution of overall TCR-repertoire complexity following transplantation of CD34-selected peripheral blood progenitor cells from unrelated donors. During the first post-transplant year, sorted CD4/45RA, CD4/45R0, CD8/45RA and CD8/45R0 subsets were analyzed at three monthly intervals for TCR-repertoire complexity by CDR3-size-spectratyping. Skew in TCR-repertoire was observed only in early memory-type T-cells. CD4+ and CD8+ subsets differed in clonal distribution of CDR3-sizes, with rapid Gaussian normalization of bands in CD4/45R0+ T-cells. Naive T-cells displayed normal repertoire complexity and contributed significantly to skew correction. Our data provides direct evidence for an important role of de novo-maturation of naive T-cells in normalization of an initially restricted TCR-repertoire following transplantation of CD34-selected, TCD peripheral blood progenitors from unrelated donors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J.-F. Poulin, M. Sylvestre, P. Champagne, M.-L. Dion, N. Kettaf, A. Dumont, M. Lainesse, P. Fontaine, D.-C. Roy, C. Perreault, et al. Evidence for adequate thymic function but impaired naive T-cell survival following allogeneic hematopoietic stem cell transplantation in the absence of chronic graft-versus-host disease Blood, December 15, 2003; 102(13): 4600 - 4607. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020