SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on June 28, 2002; DOI 10.1182/blood-2001-11-0039. This Article Full Text (PDF) All Versions of this Article: 2001-11-0039v1 100/4/1484    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lapierre, V. Articles by Tiberghien, P. Search for Related Content PubMed PubMed Citation Articles by Lapierre, V. Articles by Tiberghien, P. Social Bookmarking                   What's this? Submitted November 27, 2001 Accepted April 3, 2002 Increased presence of anti-HLA antibodies early after allogeneic G-CSF-mobilized blood hematopoietic stem cell transplantation compared to bone marrow transplantation Valerie Lapierre*, Anne Auperin, Hakim Tayebi, Jacqueline Chabod, Philippe Saas, Mauricette Michallet, Sylvie Francois, Frederic Garban, Christine Giraud, Dominique Tramalloni, Nadia Oubouzar, Didier Baise, Matthieu Kuentz, Eric Robinet, and Pierre Tiberghien Unite de Therapie Tissulaire, Cellulaire et Genique, Etablissement Francais du Sang Bourgogne-Franche-Comte, Besancon, France; Laboratoire de Therapeutique Immuno-Moleculaire, INSERM E 0119/UPRES EA 2284, Etablissement Francais du Sang Bourgogne-Franche-Comte, Besancon, France Service d'Epidemiologie et de Biostatistique, Institut Gustave Roussy, Villejuif, France Laboratoire de Therapeutique Immuno-Moleculaire, INSERM E 0119/UPRES EA 2284, Etablissement Francais du Sang Bourgogne-Franche-Comte, Besancon, France Service d'Hematologie Clinique, Hopital Edouard Herriot, Lyon, France Service des Maladies du Sang, Centre Hospitalier Universitaire, Angers, France Service d'Hematologie Clinique, Centre Hospitalier Universitaire, Grenoble, France Service d'Oncologie Hematologique et Therapie Cellulaire, Centre Hospitalier Universitaire et Etablissement Francais du Sang, Poitiers, France Unite de Medecine Transfusionnelle et d'Hemovigilance, Institut Gustave Roussy, Villejuif, France Unite de Transplantation Medullaire, Institut Paoli Calmettes, Marseille, France Service d'Hematologie, Hopital Henri Mondor, Creteil, France * Corresponding author; email: valerie.lapierre{at}efs.sante.fr. We have recently shown that the use of allogeneic G-CSF-mobilized peripheral blood hematopoietic stem cell transplantation (PBHSCT) compared with bone marrow transplantation (BMT) is associated with increased titers of antibodies (Ab) directed against red blood cell ABO antigens (Ag). To further evaluate the influence of a G-CSF mobilized PBHSC graft on allo-immune Ab responses, we examined the frequency of anti-HLA Ab after transplantation in the setting of the same randomized study comparing PBHSCT vs BMT in adults. Anti-HLA Ab presence was determined by complement-dependent lymphocytotoxicity (CDC) and flow-cytometry in the recipient before and 30 days after transplantation as well as in the donor. The use of PBHSCT was significantly associated with an increased detection of anti-HLA IgG Ab early after transplantation as evidenced by flow-cytometry (11/24 vs 4/27, p=0.03) and, less so, by CDC (5/24 vs 1/27, p=0.09). Such a difference between PBHSCT and BMT was further heightened when analysis was restricted to anti-HLA IgG Ab-negative donor/recipient pairs. In such a setting, early anti-HLA Ab was never detected after BMT while repeatedly detected after PBHSCT (flow-cytometry: 6/18 vs 0/17, p=0.02; CDC: 4/23 vs 0/26, p=0.04). Importantly, the PBHSCT-associated increase in anti-HLA Ab detection was observed despite a reduction in the median number of platelet transfusion episodes/patient in PBHSCT vs BMT recipients (3[1-21] vs 6 [3-33], p=0.02). In conclusion, our study strongly suggests that G-CSF-mobilized PBHSCT results in an increased incidence of circulating anti-HLA-Ab and further confirms that the use of such a graft alters allo-immune Ab responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Sundin, O. Ringden, B. Sundberg, S. Nava, C. Gotherstrom, and K. Le Blanc No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients Haematologica, September 1, 2007; 92(9): 1208 - 1215. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020