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Prepublished online as a Blood First Edition Paper on May 17, 2002; DOI 10.1182/blood-2001-11-0042. This Article Full Text (PDF) All Versions of this Article: 2001-11-0042v1 100/3/823    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sandrin, V. Articles by Cosset, F.-L. Search for Related Content PubMed PubMed Citation Articles by Sandrin, V. Articles by Cosset, F.-L. Social Bookmarking                   What's this? Submitted November 26, 2001 Accepted March 27, 2002 Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and non-human primates Virginie Sandrin, Bertrand Boson, Patrick Salmon, Wilfried Gay, Didier Negre, Roger Le Grand, Didier Trono, and Francois-Loic Cosset* Vectorologie Retrovirale et Therapie Genique, INSERM U412 - ENS de Lyon - IFR 74, Lyon Cedex 07, France Department of Genetics and Microbiology, Faculty of Medecine, University of Geneva, Geneva, Switzerland Service de Neurologie, CEA - CRSSA, Fontenay aux Roses, France * Corresponding author; email: flcosset{at}ens-lyon.fr. Generating lentiviral vectors pseudotyped with different viral glycoproteins (GPs) may modulate the physico-chemical properties of the vectors, their interaction with the host immune system and their host-range. We have investigated the capacity of a panel of glycoproteins of both retroviral (MLV-A, amphotropic murine leukemia virus; GALV, gibbon ape leukemia virus, RD114 feline endogenous virus) and non-retroviral (FPV, fowl plague virus; Ebola virus; VSV, vesicular stomatitis virus; LCMV, lymphocytic choriomeningitis virus) origins to pseudotype lentiviral vectors derived from simian immunodeficiency virus (SIVmac251). SIV vectors were efficiently pseudotyped with the FPV hemagglutinin, VSV-G, LCMV and MLV-A GPs. In contrast, the GALV and RD114 GPs conferred much lower infectivity to the vectors. Capitalizing on the conservation of some structural features in the transmembrane domains and cytoplasmic tails of the incorporation-competent MLV-A glycoprotein and in RD114 and GALV GPs, we generated chimeric GPs encoding the extracellular and transmembrane domains of GALV or RD114 GPs fused to the cytoplasmic tail (designated TR) of MLV-A GP. Importantly, SIV-derived vectors pseudotyped with these GALV/TR and RD114/TR GP chimeras had significantly higher titers than vectors coated with the parental GPs. Additionally, RD114/TR-pseudotyped vectors were efficiently concentrated and were resistant to inactivation induced by the complement of both human and macaque sera, indicating that modified RD114 GP-pseudotyped lentiviral vectors may be of particular interest for in vivo gene transfer applications. Furthermore, as compared to vectors pseudotyped with other retroviral GPs or with VSV-G, RD114/TR-pseudotyped vectors showed augmented transduction of human and macaque primary blood lymphocytes and CD34+ cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Verhoeyen and F.-L. Cosset Engineering the Surface Glycoproteins of Lentiviral Vectors for Targeted Gene Transfer CSH Protocols, August 1, 2009; 2009(8): pdb.top59 - pdb.top59. 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