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Prepublished online as a Blood First Edition Paper on May 17, 2002; DOI 10.1182/blood-2002-01-0035. This Article Full Text (PDF) All Versions of this Article: 2002-01-0035v1 100/4/1185    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sloand, E. M Articles by Young, N. S Search for Related Content PubMed PubMed Citation Articles by Sloand, E. M Articles by Young, N. S Social Bookmarking                   What's this? Submitted January 8, 2002 Accepted March 20, 2002 INTRACELLULAR INTERFERON- (IFN-) IN CIRCULATING AND MARROW T CELLS DETECTED BY FLOW CYTOMETRY AND THE RESPONSE TO IMMUNOSUPPRESSIVE THERAPY IN PATIENTS WITH APLASTIC ANEMIA Elaine M Sloand*, Sonnie Kim, Jaroslaw Maciejewski, John Tisdale, Dean Follman, and Neal S Young Hematology Branch, National Institutes of Health, Bethesda, MD, USA * Corresponding author; email: sloande{at}nih.gov. Immunosuppressive therapy leads to meaningful hematologic improvement in most patients with aplastic anemia (AA). Failure to respond and later relapse could be due to deficient numbers of hematopoietic stem cells, inadequate treatment of the immune process, or a non-immunologic etiology. Interferon- (IFN-) has been implicated in the pathophysiology of hematopoietic failure in AA. Based on previous findings showing over-expression of IFN- in bone marrow (BM) and peripheral blood (PB) in this disease, we hypothesized that quantitation of IFN-might be applied to predict and monitor responses to immunosuppressive therapy. We measured expression of IFN- in lymphocytes obtained from 123 patients AA, using intracellular two color fluorescent staining and flow cytometry. Thirty-six of seventy patients (51%) with severe AA demonstrated increased IFN- in circulating T cells. IFN- was detected in only four of 53 patients with recovered AA. IFN- was not found in PB lymphocytes of patients with other hematologic diseases and heavy transfusion burdens or in healthy volunteers. Among 62 AA patients who were assessed before first treatment with immunosuppressive drugs, 27/28 (96%) with circulating IFN--containing T cells subsequently responded to therapy; in contrast, only 11/34 (32%) of patients whose PB lacked IFN- lymphocytes improved to transfusion- independence. IFN--containing lymphocytes declined following treatment in all cases. Of 17 patients assessed during relapse, IFN- was present in T cells prior to the blood count decline in 13, and 12 responded to re-institution of immunosuppressive drugs. Of 30 BMs tested prior to first treatment, 20 were positive for IFN-, all in responding patients, while the negative tests were obtained in ten non-responding patients. IFN- is increased in PB lymphocyte of many patients with AA and these cells decline with therapy. The presence of intracellular IFN- may predict response to immunosuppressive treatment and also the onset of relapse. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. O. Omokaro, M. J. Desierto, M. A. Eckhaus, F. M. Ellison, J. Chen, and N. S. Young Lymphocytes with Aberrant Expression of Fas or Fas Ligand Attenuate Immune Bone Marrow Failure in a Mouse Model J. 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