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Prepublished online as a Blood First Edition Paper on April 30, 2002; DOI 10.1182/blood-2002-01-0220. This Article Full Text (PDF) All Versions of this Article: 2002-01-0220v1 100/3/862    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Humphries, R. K Articles by Hogge, D. E Search for Related Content PubMed PubMed Citation Articles by Humphries, R. K Articles by Hogge, D. E Social Bookmarking                   What's this? Submitted January 25, 2002 Accepted March 29, 2002 Deregulated expression of HOXB4 enhances the primitive growth activity of human hematopoietic cells R. K Humphries*, Christian Buske, Michaela Feuring-Buske, Carolina Abramovich, Karsten Spiekermann, Connie J Eaves, Laure Coulombel, Guy Sauvageau, and Donna E Hogge Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada; Dept. of Medicine, University of British Columbia, Vancouver, BC, Canada Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada; GSF-National Research Center for Environment and Health, and Dept. of Medicine III, Ludwigs-Maximilian University, Munich, Germany Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada GSF-National Research Center for Environment and Health, and Dept. of Medicine III, Ludwigs-Maximilian University, Munich, Germany Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada; Dept. of Medical Genetics, University of British Columbia, Vancouver, BC, Canada Faculte de Medecine, INSERM U421, Creteil, France Laboratory of Molecular Genetics of Hemopoietic Stem Cells, Clinical Research Institute of Montreal, Montreal, QC, Canada * Corresponding author; email: khumphri{at}bccancer.bc.ca. Identification of the molecular mechanisms that can promote human hematopoietic stem cell amplification is a major goal in experimental and clinical hematology. Recent data indicate that a variety of regulatory molecules active in early development may also play a role in the maintenance of hematopoietic stem cells with repopulating activity. One important class of early developmental genes determining hematopoietic development are homeobox transcription factors. Here, we report that retrovirally-mediated expression of the homeobox gene HOXB4 rapidly triggers an increase in the number of human hematopoietic cord blood cells with stem cell and progenitor cell properties detected both by in vitro and in vivo assays. This growth enhancement extended across primitive myeloid-erythroid and B-lymphoid progenitors but did not lead to alterations in the balance of lympho-myeloid reconstitution in vivo suggesting that HOXB4 does not impact control of end-cell output. These findings reveal HOXB4 as a novel, positive regulator of the primitive growth activity of human hematopoietic progenitor cells and underline the relevance of early developmental factors for stem cell fate decisions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. D. Milsom, B. Schiedlmeier, J. Bailey, M.-O. Kim, D. Li, M. Jansen, A. M. Ali, M. Kirby, C. Baum, L. J. Fairbairn, et al. Ectopic HOXB4 overcomes the inhibitory effect of tumor necrosis factor-{alpha} on Fanconi anemia hematopoietic stem and progenitor cells Blood, May 21, 2009; 113(21): 5111 - 5120. [Abstract] [Full Text] [PDF] J. Faber, A. V. Krivtsov, M. C. Stubbs, R. Wright, T. N. Davis, M. van den Heuvel-Eibrink, C. M. Zwaan, A. L. Kung, and S. A. Armstrong HOXA9 is required for survival in human MLL-rearranged acute leukemias Blood, March 12, 2009; 113(11): 2375 - 2385. [Abstract] [Full Text] [PDF] B. S. Ajjappala, Y.-S. 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