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Prepublished online as a Blood First Edition Paper on June 28, 2002; DOI 10.1182/blood-2002-01-0281. This Article Full Text (PDF) All Versions of this Article: 2002-01-0281v1 100/3/1072    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ziegler, S. Articles by Skoda, R. C Search for Related Content PubMed PubMed Citation Articles by Ziegler, S. Articles by Skoda, R. C Social Bookmarking                   What's this? Submitted January 30, 2002 Accepted March 21, 2002 A 2 kb c-mpl promoter fragment is sufficient to direct expression to the megakaryocytic lineage and sites of embryonic hematopoiesis in transgenic mice Sandra Ziegler, Kurt Burki, and Radek C Skoda* Biozentrum, University of Basel, Basel, Switzerland Institute for Laboratory Animal Sciences, University of Zurich, Zurich, Switzerland Biozentrum, University of Basel, Basel, Switzerland; German Cancer research Center (DKFZ), Heidelberg, Germany * Corresponding author; email: radek.skoda{at}unibas.ch. The thrombopoietin receptor, c-mpl, is expressed on hematopoietic progenitors and cells of the megakaryocytic lineage. The c-mpl promoter may therefore be very useful for directing expression of transgenes. We tested whether a 2 kb genomic DNA fragment comprising the putative c-mpl regulatory elements and most of the 5'-untranslated region of mouse c-mpl is able to direct expression of a reporter gene to hematopoietic cells in transgenic mice. As a reporter gene we used the human placental alkaline phosphatase (PLAP). In adult transgenic mice, PLAP expression was specifically detected in megakaryocytes and platelets. Embryos showed PLAP reporter gene expression already in the yolk sac at embryonic day 6.5 (E6.5) and in blood islands at E7.5. At E9.5 expression was found in blood vessels of the yolk sac and the embryo proper followed by high levels of expression in the fetal liver at E11.5. Expression in E6.5 yolk sac is compatible with a function of c-mpl and its ligand TPO in the earliest stages of embryonic hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. N. Constantinescu Mpl and thrombocytosis: levels matter Blood, February 19, 2009; 113(8): 1617 - 1618. [Full Text] [PDF] B. J. Lannutti, A. Epp, J. Roy, J. Chen, and N. C. Josephson Incomplete restoration of Mpl expression in the mpl-/- mouse produces partial correction of the stem cell-repopulating defect and paradoxical thrombocytosis Blood, February 19, 2009; 113(8): 1778 - 1785. [Abstract] [Full Text] [PDF] R. Tiedt, J. Coers, S. Ziegler, A. Wiestner, H. Hao-Shen, C. Bornmann, J. Schenkel, S. Karakhanova, F. J. de Sauvage, C. W. Jackson, et al. Pronounced thrombocytosis in transgenic mice expressing reduced levels of Mpl in platelets and terminally differentiated megakaryocytes Blood, February 19, 2009; 113(8): 1768 - 1777. [Abstract] [Full Text] [PDF] L. Petit-Cocault, C. Volle-Challier, M. Fleury, B. Peault, and M. Souyri Dual role of Mpl receptor during the establishment of definitive hematopoiesis Development, August 15, 2007; 134(16): 3031 - 3040. [Abstract] [Full Text] [PDF] B. Gottgens, C. Broccardo, M.-J. Sanchez, S. Deveaux, G. Murphy, J. R. Gothert, E. Kotsopoulou, S. Kinston, L. Delaney, S. Piltz, et al. The scl +18/19 Stem Cell Enhancer Is Not Required for Hematopoiesis: Identification of a 5' Bifunctional Hematopoietic-Endothelial Enhancer Bound by Fli-1 and Elf-1 Mol. Cell. Biol., March 1, 2004; 24(5): 1870 - 1883. [Abstract] [Full Text] [PDF]

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