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Prepublished online as a Blood First Edition Paper on August 15, 2002; DOI 10.1182/blood-2002-02-0355. This Article Full Text (PDF) All Versions of this Article: 2002-02-0355v1 100/13/4410    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Radomska, H. S Articles by Huettner, C. S Search for Related Content PubMed PubMed Citation Articles by Radomska, H. S Articles by Huettner, C. S Social Bookmarking                   What's this? Submitted February 4, 2002 Accepted June 27, 2002 Transgenic targeting with regulatory elements of the human CD34 gene Hanna S Radomska, David A Gonzalez, Yutaka Okuno, Hiromi Iwasaki, Andras Nagy, Koichi Akashi, Daniel G Tenen*, and Claudia S Huettner Harvard Institutes of Medicine, Harvard Medical School, Boston, MA, USA Cancer Immunology and Aids, Dana Farber Cancer Institute, Boston, MA, USA The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada * Corresponding author; email: dtenen{at}caregroup.harvard.edu. The human CD34 gene is expressed on early progenitor and stem cells in the bone marrow. Here we report the isolation of the human CD34 locus from a human PAC library and the characterization and evaluation of this genomic fragment for expression of reporter genes in stable cell lines and transgenic mice. We show that a 160 kb fragment spanning 110 kb of the 5' flanking region and 26 kb of the 3' flanking regions of the CD34 gene directs expression of the human CD34 gene in the bone marrow of transgenic mice. The expression of human CD34 transgenic RNA in tissues was found to be similar to that of the endogenous murine CD34 gene. Colony forming cell assays showed that bone marrow cells staining positive for human CD34 consist of early progenitor cells in which expression of CD34 decreased with cell maturation. In order to test the construct for its ability to express heterologous genes in vivo, we used homologous recombination in bacteria to insert the tetracycline responsive transactivator protein tTA. Analysis of transgenic human CD34-tTA mice by cross breeding with a strain carrying Cre recombinase under control of a tetracycline responsive element demonstrated induction of Cre expression in mice in a pattern consistent with the expression of the human CD34 transgene. 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