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Prepublished online as a Blood First Edition Paper on September 19, 2002; DOI 10.1182/blood-2002-02-0438. This Article Full Text (PDF) All Versions of this Article: 2002-02-0438v1 101/3/1015    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schotte, R. Articles by Spits, H. Search for Related Content PubMed PubMed Citation Articles by Schotte, R. Articles by Spits, H. Social Bookmarking                   What's this? Submitted February 8, 2002 Accepted September 4, 2002 The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T, B, and NK cell development Remko Schotte, Marie-Clotilde Rissoan, Nathalie Bendriss-Vermare, Jean-Michel Bridon, Thomas Duhen, Kees Weijer, Francine Briere, and Hergen Spits* Division of Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands Laboratory for Immunological Research, Schering Plough, Dardilly, France * Corresponding author; email: h.spits{at}nki.nl. Human plasmacytoid DC also called type 2 dendritic cell precursors or natural IFN-producing cells represent a cell type with distinctive phenotypic and functional features. They are present in the thymus and probably share a common precursor with T and NK cells. In an effort to identify genes that control pDC development we searched for genes of which the expression is restricted to human pDC using a cDNA subtraction technique with activated monocyte-derived DC as competitor. We identified the transcription factor Spi-B to be expressed in pDC but not in Mo-DC. Spi-B expression in pDC was maintained upon in vitro-maturation of pDC. Spi-B was expressed in early CD34+CD38- hematopoietic progenitors and in CD34+CD1a- thymic precursors. Spi-B expression is downregulated when uncommitted CD34+CD1a- thymic precursors differentiate into committed CD34+CD1a+ pre T cells. Overexpression of Spi-B in hematopoietic progenitor cells resulted in inhibition of development of T cells both in vitro and in vivo. In addition, development of progenitor cells into B and NK cells in vitro was also inhibited by Spi-B overexpression. Our results indicate that Spi-B is involved in the control of pDC development by limiting the capacity of progenitor cells to develop into other lymphoid lineages. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Hochmeister, M. Zeitelhofer, J. Bauer, E.-M. Nicolussi, M.-T. Fischer, B. Heinke, E. Selzer, H. Lassmann, and M. Bradl After Injection into the Striatum, in Vitro-Differentiated Microglia- and Bone Marrow-Derived Dendritic Cells Can Leave the Central Nervous System via the Blood Stream Am. J. Pathol., December 1, 2008; 173(6): 1669 - 1681. [Abstract] [Full Text] [PDF] H. Schmidlin, S. A. Diehl, M. Nagasawa, F. A. Scheeren, R. Schotte, C. H. Uittenbogaart, H. Spits, and B. Blom Spi-B inhibits human plasma cell differentiation by repressing BLIMP1 and XBP-1 expression Blood, September 1, 2008; 112(5): 1804 - 1812. [Abstract] [Full Text] [PDF] G. Lenz, I. Nagel, R. Siebert, A. V. Roschke, W. 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