SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on June 14, 2002; DOI 10.1182/blood-2002-02-0445. This Article Full Text (PDF) All Versions of this Article: 2002-02-0445v1 100/8/2899    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Howie, D. Articles by Terhorst, C. Search for Related Content PubMed PubMed Citation Articles by Howie, D. Articles by Terhorst, C. Social Bookmarking                   What's this? Submitted February 14, 2002 Accepted June 3, 2002 The role of SAP in murine CD150 (SLAM)-mediated T cell proliferation and Interferon gamma production Duncan Howie*, Susumo Okamoto, Svend Rietdijk, Kareem Clarke, Ninghai Wang, Charles Gullo, Joost P Bruggeman, Stephen Manning, Anthony J Coyle, Edward Greenfield, Vijay Kuchroo, and Cox Terhorst Division of Immunology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA Inflammation Division, Millenium Pharmaceuticals Inc, Cambridge, MA, USA Department of Adult Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA, USA Department of Neurology, Center for Neurologic Diseases, Brigham and Womens Hospital and Harvard Medical School, Boston, MA, USA * Corresponding author; email: dhowie{at}caregroup.harvard.edu. CD150 (SLAM) is a self-ligand cell surface glycoprotein expressed on T cells, B cells, macrophages and dendritic cells. To further explore the role of CD150 signaling in co-stimulation and TH1 priming we have generated a panel of rat anti-mouse CD150 monoclonal antibodies. CD150 cell-surface expression is upregulated with rapid kinetics in activated T cells and LPS/Interferon gamma activated macrophages. Anti-CD150 triggering induces strong co-stimulation of T cells triggered through CD3. DNA synthesis of murine T cells induced by anti-CD150 is not dependent on SLAM associated protein (SAP, SH2D1A), as anti-CD150 induces similar levels of DNA synthesis in SAP-/- T cells. Antibodies to CD150 also enhance Interferon gamma production both in wild-type and SAP-/- T cells during primary stimulation. The level of Interferon gamma production is higher in SAP-/- T cells than in wild type T cells. Anti-CD150 antibodies also synergize with IL-12 treatment in up-regulation of IL-12 receptor ß2 mRNA during TH1 priming, and inhibit primary TH2 polarization in an Interferon gamma-dependent fashion. Crosslinking CD150 on CD4 T cells induces rapid serine-phosphorylation of Akt/PKB. We speculate that this is an important pathway contributing to CD150 mediated T cell proliferation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. A. Jordan, J. M. Fletcher, D. Pellicci, and A. G. Baxter Slamf1, the NKT Cell Control Gene Nkt1 J. Immunol., February 1, 2007; 178(3): 1618 - 1627. [Abstract] [Full Text] [PDF] M. M. McCausland, I. Yusuf, H. Tran, N. Ono, Y. Yanagi, and S. Crotty SAP Regulation of Follicular Helper CD4 T Cell Development and Humoral Immunity Is Independent of SLAM and Fyn Kinase J. Immunol., January 15, 2007; 178(2): 817 - 828. [Abstract] [Full Text] [PDF] S. Crotty, M. M. McCausland, R. D. Aubert, E. J. Wherry, and R. Ahmed Hypogammaglobulinemia and exacerbated CD8 T-cell-mediated immunopathology in SAP-deficient mice with chronic LCMV infection mimics human XLP disease Blood, November 1, 2006; 108(9): 3085 - 3093. [Abstract] [Full Text] [PDF] N. Wang, M. Campo, L. Ting, C. Fleming, C. Terhorst, and P. W. Finn The Costimulatory Molecule SLAM Is Critical for Pulmonary Allergic Responses Am. J. Respir. Cell Mol. Biol., August 1, 2006; 35(2): 206 - 210. [Abstract] [Full Text] [PDF] A.-C. Rocha-Campos, R. Melki, R. Zhu, N. Deruytter, D. Damotte, M. Dy, A. Herbelin, and H.-J. Garchon Genetic and Functional Analysis of the Nkt1 Locus Using Congenic NOD Mice: Improved V{alpha}14-NKT Cell Performance but Failure to Protect Against Type 1 Diabetes. Diabetes, April 1, 2006; 55(4): 1163 - 1170. [Abstract] [Full Text] [PDF] B. Rethi, P. Gogolak, I. Szatmari, A. Veres, E. Erdos, L. Nagy, E. Rajnavolgyi, C. Terhorst, and A. Lanyi SLAM/SLAM interactions inhibit CD40-induced production of inflammatory cytokines in monocyte-derived dendritic cells Blood, April 1, 2006; 107(7): 2821 - 2829. [Abstract] [Full Text] [PDF] H.-C. Chuang, J.-D. Lay, W.-C. Hsieh, H.-C. Wang, Y. Chang, S.-E. Chuang, and I.-J. Su Epstein-Barr virus LMP1 inhibits the expression of SAP gene and upregulates Th1 cytokines in the pathogenesis of hemophagocytic syndrome Blood, November 1, 2005; 106(9): 3090 - 3096. [Abstract] [Full Text] [PDF] D. Howie, F. S. Laroux, M. Morra, A. R. Satoskar, L. E. Rosas, W. A. Faubion, A. Julien, S. Rietdijk, A. J. Coyle, C. Fraser, et al. Cutting Edge: The SLAM Family Receptor Ly108 Controls T Cell and Neutrophil Functions J. Immunol., May 15, 2005; 174(10): 5931 - 5935. [Abstract] [Full Text] [PDF] C. Farina, D. Theil, B. Semlinger, R. Hohlfeld, and E. Meinl Distinct responses of monocytes to Toll-like receptor ligands and inflammatory cytokines Int. Immunol., June 1, 2004; 16(6): 799 - 809. [Abstract] [Full Text] [PDF] H. Jiang, C. Van de Ven, P. Satwani, L. V. Baxi, and M. S. Cairo Differential Gene Expression Patterns by Oligonucleotide Microarray of Basal versus Lipopolysaccharide-Activated Monocytes from Cord Blood versus Adult Peripheral Blood J. Immunol., May 15, 2004; 172(10): 5870 - 5879. [Abstract] [Full Text] [PDF] A. Veillette SLAM Family Receptors Regulate Immunity with and without SAP-related Adaptors J. Exp. Med., May 3, 2004; 199(9): 1175 - 1178. [Abstract] [Full Text] [PDF] N. Wang, A. Satoskar, W. Faubion, D. Howie, S. Okamoto, S. Feske, C. Gullo, K. Clarke, M. R. Sosa, A. H. Sharpe, et al. The Cell Surface Receptor SLAM Controls T Cell and Macrophage Functions J. Exp. Med., May 3, 2004; 199(9): 1255 - 1264. [Abstract] [Full Text] [PDF] M. Simarro, A. Lanyi, D. Howie, F. Poy, J. Bruggeman, M. Choi, J. Sumegi, M. J. Eck, and C. Terhorst SAP increases FynT kinase activity and is required for phosphorylation of SLAM and Ly9 Int. Immunol., May 1, 2004; 16(5): 727 - 736. [Abstract] [Full Text] [PDF] A. Malbran, L. Belmonte, B. Ruibal-Ares, P. Bare, I. Massud, C. Parodi, M. Felippo, R. Hodinka, K. Haines, K. E. Nichols, et al. Loss of circulating CD27+ memory B cells and CCR4+ T cells occurring in association with elevated EBV loads in XLP patients surviving primary EBV infection Blood, March 1, 2004; 103(5): 1625 - 1631. [Abstract] [Full Text] [PDF] V. Pasquinelli, M. F. Quiroga, G. J. Martinez, L. C. Zorrilla, R. M. Musella, M. M. Bracco, L. Belmonte, A. Malbran, L. Fainboim, P. A. Sieling, et al. Expression of Signaling Lymphocytic Activation Molecule- Associated Protein Interrupts IFN-{gamma} Production in Human Tuberculosis J. Immunol., January 15, 2004; 172(2): 1177 - 1185. [Abstract] [Full Text] [PDF] S. G. Tangye, K. E. Nichols, N. J. Hare, and B. C. M. van de Weerdt Functional Requirements for Interactions Between CD84 and Src Homology 2 Domain-Containing Proteins and Their Contribution to Human T Cell Activation J. Immunol., September 1, 2003; 171(5): 2485 - 2495. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020